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International Seed Testing Association News Bulletin No. 150 October 2015 WWW.SEEDTEST.ORG ISTAS FIRST INDUSTRY MEMBERS DOW AGROSCIENCES AND DUPONT PIONEER 28 Seed industry steps up to contribute ISTA SEED SAMPLING CERTIFICATE 38 Newly approved changes offer another tool for accredited Members17 STRONG COMMUNICATIONS HAVE BEEN KEY TO ISTAS SUCCESS AND GROWTH 150 STI ISSUES 08 47 17 SEED TESTING INTERNATIONAL www.seedtest.org From the Editor Post-Annual-Meeting 2 Calendar ISTA events and meetings 3 Presidents Report Partners in Progress 4 Feature Articles Changing with the Times The 150th edition of the ISTA News Bulletin 6 35 ON THE COVER The first issue of Seed Testing International was printed in 1959. The News Bulletin has evolved over the years changing with the times to become a key member communications tool. CONTENTS 50 SEED TESTING INTERNATIONAL OCTOBER 2015 Association News ISTA Community Holds its 2015 Annual Meeting 8 Seminar Report Molecular Tools for Seed Quality and Seed Health 12 Auditors Meeting 2015 13 Changes to the International Rules 16 31st ISTA Congress 17 Second Call for Papers 24 Successful Start for ISTA on Social Media 26 The ISTA Hop Hornbeam in the State Arboretum in Stuttgart-Hohenheim 27 ISTAs First Industry Members Dow AgroSciences and DuPont Pioneer 28 New ISTA Members 29 Rules Development Validation Study for the Germination of Dactylis glomerata and Festuca arundinacea 30 Accreditation Accreditation of the Seed Testing Laboratory FSBI Bryanskaya MVL in Russia 35 ISTA Accreditation For Sampling Only 37 ISTA Seed Sampling Certificate 38 DuPont Pioneer Asia Pacific Regional Quality Lab 39 Results of GMO Proficiency Test 20 40 Accreditation of the FASMAC Seed Testing Laboratory Kanagawa Japan 42 Laboratory Accreditation Changes 43 Seed Science Science in ISTA A new initiative 44 Training and Education Announcements 46 Workshop Reports 48 2 SEED TESTING INTERNATIONAL www.seedtest.org Seed Testing International No. 150 October 2015 ISSN 1999-5229 Produced on behalf of the ISTA Executive Committee The views and opinions expressed by authors in this publication are not necessarily those of the International Seed Testing Association ISTA. No endorsement of any non-ISTA products or services mentioned in this publication is given or implied by the International Seed Testing Association. Chief Editor Dr. Benjamin Kaufman Editors Jonathan Taylor Pierrick Marcoux International Seed Testing Association ISTA ISTA Secretariat Zrichstrasse 50 CH-8303 Bassersdorf Switzerland Phone 41 44 838 60 00 Fax 41 44 838 60 01 E-mail ista.officeista.ch Internet www.seedtest.org Seed Testing International No. 151 Deadline for article submission 15 February 2016 Publication date April 2016 Circulation 1750 No. of copies printed 2250 Instructions to contributors www.seedtest.orgSTI Other ISTA Publications For more information on other ISTA publications please contact the ISTA Secretariat or visit our website at www.seedtest.org Follow ISTA on LinkedIn Greetings fellow seed analysts THIS IS THE POST-ANNUAL-MEETING ISSUE OF STI. This year we had a very successful meeting in Montevideo Uruguay. The organization of the event was efficient the atmosphere was relaxed and friendly it is a family event after all... and the hospitality of the Uruguayan team was wonderful this backdrop was conductive to fruitful interactions between the participants the Members the ECOM and the TCOMs. Nevertheless this Annual Meeting was not without challenges. The location was distant for many and the registration fee was relatively high compared to previous years these factors were prohibitive to many Members a fact that manifested itself in a relatively lower attendance. ISTA is an international organization therefore we ought to provide all our members around the globe with the opportunity of having periodically an Annual Meeting in their part of the world and hope that they will take advantage of the occasion. This time it was South America I am glad that we had the opportunity to visit Uruguay and learn more about the country and its seed sector. An important component of the success of this annual event is the decisions that were made. I would like to highlight some that I believe to be a step change for our Association. Accreditation for sampling only and the ISTA Sampling Certificate this work led by the ECOM Accreditation Working Group with the help of the Accreditation and Technical Department at the Secretariat involved revisiting the definition of a laboratory and finding a new definition for bodies dedicated to sampling only now called sampling entities The differentiation of laboratory and sampling entity called for updating the ISTA Accreditation Standard which was done and approved. This whole initiative prepared the ground for the creation of the ISTA Sampling Certificate of which a draft was presented at the Annual Meeting. This latest step in the evolution of our Accreditation System answers a need that was expressed by the seed sector. It differentiates and emphasizes the importance of proper sampling in seed testing and allows greater operational agility for the seed industry and the laboratories and analysts involved in it. Following up on their earlier agreement in principle to employ Contracted System Auditors Feb. 2015 see April 2015 STI editorial the ECOM approved at the Meeting in Montevideo all the instruments required to implement this new mode of operation for the Accreditation Department. In parallel to recruiting System Auditors we have also launched an effort to engage more Technical Auditors. The newly approved contract and payment scheme will hopefully help us in our effort to make these functions attractive to qualified seed analysts and ease their recruitment. We have already engaged one contracted System Auditor Jette Nydam from Denmark who will go on her first contracted audit later this year. We also have four more Technical Auditors who have joined our pool Valerie Cockerell from the United Kingdom Lea Mazor from Israel Todd Erickson from the U.S. and our very own Florina Palada originally from Romania who is one of the ISTA System Auditors but is now also qualified as a Technical Auditor. Lastly the ECOM has approved our engagement with Issues Ink as a publishing house and web site designer. We have already started the work the results of which I hope you will see in the not too distant future in the form of an upgraded and more functional ISTA web site. However the first results of this collaboration you can already see in the present issue of STI. This issue is the first produced by Issues Ink and equally significant it is the 150th issue of the ISTA News Bulletin STI. As you can judge by the cover... Seed Testing International has been through some impressive evolutions over time and I hope you will find this latest step in its development a pleasing one. I wish you joyous reading Beni Kaufman FROM THE EDITOR 3SEED TESTING INTERNATIONAL OCTOBER 2015 CALENDAR 2015 1113 October European Seed Association Annual MeetingEuropean Seed Trade Meeting Vienna Austria httpesa.conceptum.eu 911 November ISTA Workshop on Seed Sampling and Quality Assurance in Seed Sampling Johannesburg South Africa httpwww.seedtest.org 2326 November ISTA Workshop on Seed Sampling and Quality Assurance in Seed Sampling Johannesburg South Africa httpwww.seedtest.org 1619 November Asian Seed Congress Goa India httpapsaseed.org 2016 13 March AFSTA African Seed Trade Association Congress 2016 Nairobi Kenya httpafsta.org 1518 May ISF World Seed Congress Punta del Este Uruguay httpworldseedcongress2016.com 1013 June ISTA Quality Assurance Workshop for Advanced Laboratories Saku Estonia httpwww.seedtest.org 1013 June ISTA Workshop on Germination Testing Saku Estonia httpwww.seedtest.org 1521 June 31st ISTA Congress Tallinn Estonia httpista-tallinn2016.ee 201516 Calendar For seed companies independent seed labs and plant scientists who need precisely controlled environments and long-term reliability Conviron provides High throughput seed germinator Multi-purpose incubator Seed storage rooms Custom solutions Visit www.conviron.comseed to learn more. C H A M B E R S R O O M S SEEDSEED G1000 Seed Germinator Conviron is the world leader in controlled environment systems for plant science research. 4 SEED TESTING INTERNATIONAL www.seedtest.org Partners in ProgressJol Lchapp PRESIDENTS REPORT FROM 15-18 JUNE 2015 ISTA held its Annual Meeting in Montevideo the capital of Uruguay at the invitation of Mr. Tabar Aguerre Minister for Livestock Agriculture and Fisheries of the Rpublica Oriental del Uruguay. This was the fourth time in 23 years that ISTA has had the pleasure of holding its Annual Meeting in South America. Previous meetings were held in Argentina in 1992 Bolivia in 2002 and Brazil in 2007. There is no doubt that South America is a very important area for seed production. ISTA is very proud to have so many active members from South American countries including those in Uruguay. Mr. Tabar Aguerre in his presentation demonstrated how Uruguayan governmental policies on seeds and varieties were supporting the seed industry. In 1997 Uruguay implemented a very intensive programme for seed production and strengthened its seed regulatory organization by the creation of INASE Instituto Nacional de Semillas. On behalf of ISTA and all participants I would like to thank Minister Aguerre and the National Organizing Committee INASE comprising Pedro Queheille President of INASE Daniel Bayce Director Vanessa Sosa Torres Director of the Seed Quality Laboratory Maria Ines Fornos Director of Communication Cecilia Jones ISTA Executive Committee member 2013-2015 and former Director of INASE who hosted and organized the 2015 Annual Meeting and all staff of INASE. The excellent organization and the high quality of the facilities resulted in a very constructive and friendly meeting contributing to a very successful meeting. The scientific and technical programme of the 2015 Annual Meeting started with two workshops and was followed by a seminar on Molecular tools applied to seed quality and seed health on Monday 15 June opening the official programme of the Annual Meeting. The presentations of the Technical Committees summarized the significant progress that these Committees have made the one year since the 2014 Annual Meeting. The work of the Technical Committees is the heart of ISTA. ISTA has around 225 committee members all of whom have great expertise and scientific knowledge and who give many hours of their time to the work of ISTA. Some are able to do this in their working time and for this I sincerely thank their employers. Others do ISTA work in their own time on a voluntary basis and I extend my warmest thanks to them also. The work presented by the technical committees is of outstanding quality and reflects the expertise of the members of the committees. They propose new rules every year and did so again this year. These Rules Proposals are validated following the robust method validation programme which includes the review by external experts and accurate statistical analyses supported by the Statistics Committee. This is the strength of our Association. It guarantees the international reliability of the ISTA Rules. I invite you to consult the method validation reports on the ISTA website and to see the new edition of the Rules when it comes into force on 1 January 2016. During this Annual Meeting the most recent changes to the accreditation programme were presented. They are designed to better fit the needs of the seed sector. Accreditation has been extended to include sampling by entities other than laboratories. An ISTA sampling certificate has been proposed as an experiment to facilitate the link between the sampling on the lot and the testing laboratories. This will allow sampling only entities in many countries to become ISTA accredited and enable use of the ISTA methods for sampling for purposes other than just seed quality assessment. For example ISTA sampling methods for seed could be used to sample for quarantine pest control or for seed testing for national certification schemes. This may also contribute to solutions for better monitoring of networks of samplers when samplers are isolated and spread through large countries or are in different countries under the same sampling authority. For this new area of accreditation for sampling only entities in addition to laboratories the applications may extend beyond any foreseen expectations. The intention was always to increase the uniformity of sampling and in so doing increase the uniformity of seed testing worldwide. Following the views expressed by the membership at the 2014 Ordinary General Meeting in Edinburgh the accreditation audits fees have been restructured to spread more evenly the payment of the fees. In addition after the positive opinion expressed by the membership in 2014 at the Edinburgh OGM the Executive Committee has implemented a five per cent discount for early payment of the membership fees. The combination of both decisions results in facilitation of budgeting for the laboratories and a decrease by five per cent of membership fees accreditation fees and audit fees under condition of early payment. ISTAs financial reserves will be used to implement this decision. During this meeting the membership were informed of the major challenge that ISTA now faces in the light of the European Union regulations Number 7652008. According to this regulation ISTA accreditation could no longer be possible in the EU territory or alternatively ISTA can no longer use the wording accreditation for accredited laboratories. Not recognizing ISTA accreditation in the EU for regulatory analysis of seed will potentially 5SEED TESTING INTERNATIONAL OCTOBER 2015 jeopardize seed export from the European states to the rest of the world. place European laboratories in a weaken competitive position vis--vis other countries outside Europe where ISTA accreditation is recognized or compulsory for import of seeds impose a double accreditation ISO 17025 and ISTA on EU laboratories ISO accreditation for regulatory analyses such as seed certification ISTA accreditation for export as recommended by the international trade have an impact beyond the borders of Europe decrease worldwide harmonization of seed quality standards and testing and have major negative impacts on both the import and export of seeds to and from the EU. The ECOM is confident that ISTA gives the seed sector a high quality accreditation system that respects the regulations and is adapted to the needs of the trade and regulators. The ECOM is in close contact with the European Union. An action plan has been worked out with the contribution of a lawyer contracted by ISTA. To find a workable solution ISTA now needs the support of the stakeholders the designated authorities who vote in ISTA the seed industry and all the ISTA members to convince the EU to maintain the recognition of the ISTA accreditation system. In addition to the above topics I would like to highlight areas which illustrate the developments and the main goals within ISTA Enabling easy access to the Rules and the Certificates is a major ongoing project in ISTA. As you may be aware in 2014 the ISTA Rules became available in electronic format. This has been very successful and the electronic availability of the Rules is very much appreciated by the laboratories and all the other stakeholders. Following the demand of the membership and stakeholders ISTA is currently investigating the possibility of an electronic version of International Certificates. Another ISTA project is to increase the number of different languages in which the Rules and handbooks are available. The ECOM strongly supports the translation of the Rules into Spanish to facilitate the promotion and use of the Rules by the laboratories and the seed trade in the Spanish speaking countries. One of ISTAs key objectives is to further develop its direct collaboration with the seed industry. This is to ensure that the work undertaken by ISTA continues to respond to the industrys needs. To facilitate this in 2014 in consultation with the seed industry a new membership cate- gory the Industry membership this replaces the previous corporate membership was created which included the establishment of a Seed Industry Advisory Committee. The objectives of the Seed Industry Advisory Committee are to provide a link between the association and industry needs including but not limited to tech- nical challenges and research needs. During the 2015 Annual Meeting the seed industry strongly supported by ISF and some ISTA members expressed the needs to take into consideration the specific needs of the vegetable seed sector. To respond to this request the ECOM decided to establish a new Working Group on Vegetables. The objectives are to define the questions and priorities of the international vegetable seed industry and to recommend to the ECOM the best ways for ISTA and the vegetable industry to address these needs. This WG on vegetables will work in close consultation with the Seed Industry Advisory Committee and the ISTA committees. Another area where ISTA is currently very active is the promotion of its activities and services to the seed sector including the regulatory bodies the seed industry and the research organiza- tions. To help achieve this goal we have recruited a marketing head to the Secretariat. Pierrick Marcoux has been appointed to this position and he works in close collaboration with the ECOM working group on Marketing. Marketing and communication plans have been developed and are being implemented. A further objective of ISTA coming from the Strategy 2013-2016 is to increase scientific input to support the development of new methodolo- gies and techniques in seed testing. To achieve this goal a Seed Science Advisory Group SSAG has been created. I invite you to read Science in ISTA A new initiative formation of the Seed Science Advisory Group by Dr. Alison Powell ISTA Honorary Life Member and Chair of the SSAG. It describes clearly the goals and role of the SSAG in ISTA. The Industry Advisory Group and the Seed Science Advisory Group were set up in 2014 to strengthen the work of ISTA in the major work areas of ISTA. They help ISTA to reinforce the link between basic research ongoing developments in the seed industry and the technical developments made by the ISTA Technical Committees. Although these areas are not new to ISTA the structured approach offered by the Science Advisory Group and the Industry Advisory Group together with the Technical Committees create a very strong foundation which will ensure the relevance of future developments in ISTA. I would like to remind you that it is now less than seven months before the Congress in Tal- linn Estonia when the Technical Committees and the Executive Committee will be renewed. I invite you all to consider this opportunity for you or colleagues within your organization to strengthen your engagement within ISTA. If you would like to be a candidate for the ECOM or a Technical Committee Member and would like any more information on this subject please feel free to contact the Secretariat the commit- tee chairs or any ECOM member. The ECOM will prepare the proposed strategy to be voted at the OGM during the 31st Congress next year in Tallinn. The ECOM wishes to consult with the Technical Committees and with all Members and stakeholders. To enable you to share your ideas a questionnaire will be sent to you toward the end of the year. Any contribution to the preparation of the new ISTA strategy is welcome. Please send your proposals to the both me as the President and Craig McGill as Vice-President. This renewed strategy will be implemented under the Presidency of Craig. To conclude I would like to thank everyone who has supported the work of ISTA in 2014- 2015. Thanks go to the Executive Committee Members to the Technical Committees and to the ISTA Secretariat who not only do such a good job on a daily basis but who are also covering for currently vacant positions in the Secretariat. Mythoughtsgoalsotothoseofyouwhoarededicat- edworkersforISTAandthatforpersonalandhealth reasonhavetofaceperiodsofabsence.Iwishyou agoodrecoveryandawelcomebackwhenyouare readytorejointheAssociationonceagain. The Executive Committee together with the Estonian Organizing Committee represented by Margus Friedenthal have the great pleasure of inviting you to our 31st Congress in Tallinn in Estonia next year. Your President and ISTA colleague Jol Lchapp 6 SEED TESTING INTERNATIONAL www.seedtest.org Changing with the Times The 150th edition of the ISTA News BulletinJonathan Taylor FEATURE ARTICLE ISTA Publications Unit ISTA Secretariat 8303 Basserdorf Switzerland jonathan.taylorista.ch IT WAS IN AUGUST 1959 WHEN THE ISTA NEWS BULLETIN NB MADE ITS FIRST APPEARANCE AS VOLUME I NO. 1. Its first editor was the new ISTA SecretaryTrea- surer Dr. Akker Schoorel of the Seed Testing Sta- tion at Wageningen the Netherlands. In those days the ISTA Secretariat was located wherever the SecretaryTreasurer happened to have his office. Dr. Schoorel had been newly elected at the 12th International Seed Testing Convention in Oslo Norway the previous month. On the subject of the Convention the first News Bulletin contains the following item 4. The Convention. The chairman of the Program Committee per- haps Dr. Schoorel himself -JT managed to press together 67 profound studies in less than three days without losing his temper nor that of the participants apologizes for this English. Oslo proved that I.S.T.A. is a talkative organization. The News Bulletin was most probably the brain- child of the new ISTA President Prof. P.A. Line- han of Queens University Belfast UK as Dr. Schoorel wrote in April 1976 49 issues later in No. 50. By then the News Bulletin had evolved from several thin green A4 sheets stapled together in one corner Fig. 1 to several thicker blue sheets now printed on both sides and with a special cover sheet but still stapled together in one corner Fig. 2. This format had been introduced in 1965 with issue No. 16. For almost 30 years this was how the News Bulletin appeared Fig. 3 until 1994 with No. 107 when it changed to a brochure format with folded double sheets and stapled along the spine and became a proper maga- zine Fig. 4.FIGURE 1. News Bulletin 001 August 1959. 7SEED TESTING INTERNATIONAL OCTOBER 2015 It is also worth mentioning that for many years during this period the News Bulletin was pub- lished not only in English but also in French German and Spanish translated with the help of ISTA colleagues who were native speakers of those languages. Changes then began to speed up. In October 1999 No. 120 was on white glossy paper Fig. 5 to allow colour printing especially for adverts. Then in 2003 with issue No. 125 came not only a new cover with four-colour printing throughout Fig. 6 but also a new title Seed Testing International. This only lasted until 2007 when the new STI received a complete makeover inside and out Fig. 7. In this form it lasted for the next eight years quite a long time for News Bulletin standards. And now we have taken another step forward with STI with a different page size a new con- cept a new printer and a new design. But still on paper as in 1959. And with ever faster progress being made in information technology what will be the next step for Seed Testing International There are already a few ideas running around my head. FIGURE 2. News Bulletin 50 April 1976. FIGURE 3. News Bulletin 100 October 1991. FIGURE 4. News Bulletin 107 December 1994. FIGURE 5. News Bulletin 120 October 1999. FIGURE 6. News Bulletin 125 April 2003. FIGURE 7. News News Bulletin 133 April 2007. 8 SEED TESTING INTERNATIONAL www.seedtest.org ASSOCIATION NEWS ISTA Community Holds its 2015 Annual Meeting MontevideoUruguay15-18June2015 THE INTERNATIONAL SEED TESTING ASSOCIATION ISTA Annual Meeting was held in Montevideo Uruguay from 15-18 June 2015 and was hosted by the National Seed Institute INASE. The main objectives of the ISTA Annual Meeting are to decide on proposals for changes to the lnternational Rules for Seed Testing and to present the work of ISTAs Technical Committees. At the opening ceremony participants were greeted by welcome speeches from Jol Lchapp and Pedro Queheille the Presidents of ISTA and INASE respectively. The meeting brought together 129 participants from 39 countries including ISTA delegates and representatives from both the seed industry and governments. To kick off the Annual Meeting a seminar was held on Molecular tools applied to Head of Marketing ISTA Secretariat 8303 Bassersdorf Switzerland pierrick.marcouxista.ch Pierrick Marcoux seed quality and seed health. The seminar provided an update on the use of molecular techniques and corresponding statistical analyses for varietal identification GMO testing and seed health. In conjunction with the Annual Meeting two ISTA Workshops also took place in Uruguay Seed Sampling and Quality Assurance in Seed Sampling and Tetrazolium testing for viability and its use as a vigour test for Glycine max providing quality training opportunities for all participants. Some of the highlights of this years meeting were the announcement that ISTA accredited laboratories and Sampling Entities will be able to issue an ISTA Seed Sampling Certificate ISSC this new Certificate will complement the existing Orange and Blue ISTA Certificates. Another important announcement was of a change in the payment schedule of the audit fee for accredited laboratories. Starting 2016 the fee will no longer be charged once every three years but instead will be invoiced in annual instalments. This change will make the budgeting for payment of the fee more convenient for the laboratories. Another development of this years annual meeting was the first use of electronic voting during the Ordinary General Meeting which replaced the old manual card raising method. On the last day of the Annual Meeting participants were given a very insightful presentation on Uruguay and the Uruguayan seed sector by Tabar Aguerre the Uruguayan Minister of Livestock Agriculture and Fisheries. Finally Lchapp concluded ISTA wants to thank INASE for a great event organization and he invited everyone to save the date for next years 31st ISTA Congress in Tallin Estonia which is scheduled for 14-21 June 2016. 9SEED TESTING INTERNATIONAL OCTOBER 2015 10 SEED TESTING INTERNATIONAL www.seedtest.org ASSOCIATION NEWS 11SEED TESTING INTERNATIONAL OCTOBER 2015 12 SEED TESTING INTERNATIONAL www.seedtest.org ASSOCIATION NEWS Seminar Report Molecular Tools for Seed Quality and Seed Health Chair ISTA Variety Committee Instituto Nacional de Semillas INASE Laboratorio de Marcadores Moleculares y Fitopatologa 1063 Buenos Aires Argentina alvicarioinase.gov.ar Ana Laura Vicario IN THE FRAMEWORK of the 2015 ISTA Annual Meeting a Seminar on Molecular Tools for Seed Quality and Seed Health was held organized by the Organizing Committee of the ISTA Annual Meeting the Seed Health Committee the GMO Committee and the Variety Committee. Twelve specialists from different countries presented the advances on several topics related to seed health statistics and molecular tools. In the first session of the morning the Chair of the Variety Committee and a Committee member Elisa Vieira presented the advances on the application of DNA-based techniques within ISTA and the state of the art for the application of molecular techniques for certification of genetic purity and varietal identification. The Vice-Chair of the GMO Committee Ren Mathis and other members of that Committee presented the New Rules Chapter and Handbook the most commonly used techniques for detection identification and quantification of GMOs Nilson Castanheira and a historical revision of the evolution of GM crop development from the 1990s to today the pipeline products and next generation GMOs and impacts on seed testing Raymond Shillito. Finally Marco Mazzara EU representative and GMO Committee member presented the organization and work of the European Network of GMO Laboratories ENGL. During the afternoon the Statistics Committee Chair Jean-Louis Laffont astonished the auditorium with a tour of statistical tools used in GMO testing presenting the Seedcalc software and the ways for computing measurement uncertainty and for using Bayesian statistics. After that the Chair of the Seed Health Committee Valrie Grimault together with other speakers Marcel Toonen Siham Assad Valerie Cockerell and Christian Barreau presented topics related with pathogen detection by means of traditional and molecular tools and the impacts and control of several seed-borne diseases on different crops. The Seminar closed with the words of the ISTA President Jol Lchapp who thanked the lecturers for the presentations and the audience for the questions raised during the day. 13SEED TESTING INTERNATIONAL OCTOBER 2015 Auditors Meeting 2015 ISTA Accreditation Department ISTA Secretariat 8303 Bassersdorf Switzerland branislava.opraista.ch Branislava Opra Audit 2014 2015 2016 1st 6 1 2nd 4 4 9 3rd 4 10 7 4th 3 3 4 5th 8 3 2 6th 11 25 28 7th - 1 5 Total 36 47 55 TABLE 1. Overview of the three-yearly cycle of ISTA audits. THE ISTA AUDITORS met on 15 June 2015 in conjunction with the ISTA Annual Meeting held in Montevideo Uruguay. Gerarda de Boer-Raatgever who joined the auditors group in 2014 and Valerie Cockerell who was present as technical auditor in training were welcomed. In 2015 the Accreditation Department will have performed 46 re-accreditation audits plus one audit of a new laboratory that asked for their first ISTA audit. For 2016 this number rises to 55 audits making it the busiest year in the three-yearly cycle of accreditation Table 1. Recruiting of New Auditors As the Accreditation Department is currently operating with only two system auditors and 10 technical auditors and the number of audits is continuously increasing it was decided to recruit new technical and system auditors who will be contracted for performing ISTA audits. For recruiting new technical auditors 20 laboratories with the widest scope of accreditation were approached. Seven candidates expressed their interest and were taken into the training and evaluation procedure. For recruiting system auditors three candidates were approached and one replied positively to the offer. A new detailed procedure has been developed for documenting the recruitment training and evaluation process Checklist for evaluation of new auditors in training with ratings one to five. The limits of acceptance are yet to be defined. Next to the technical competence for auditing the appropriate presentation of the ISTA spirit will be evaluated. New candidates will be supported by providing the opportunity of attending one or two observation audits before taking an exam audit. Candidates will undergo a trial period of three independent audits to enable both sides to gain confidence in mutual cooperation. Audit Duration 2015 The audit plan and progress was presented with related statistics and audit feedbacks. The statistics show that duration of the audit day has significantly improved over the years. An even better time management can be achieved by selecting the tests to be audited provided that the omitted tests are covered by suitable PT results and positive previous reports. The laboratory shall be informed about the selection in advance. Tests and activities that auditors could not witness during the audit shall be reported from now on in the Audit Report and the next auditors team will proceed with witnessing them at the next audit. Audit Feedback Around 60 per cent of the audited laboratories completed the feedback form after the audit Figure 1. In order to obtain FIGURE 1. Percentage of audit feedback forms submitted over the past four years. ofresponses 100.0 90.0 80.0 70.0 60.0 50.0 40.0 30.0 2011 2012 2013 2014 responses 14 SEED TESTING INTERNATIONAL www.seedtest.org ASSOCIATION NEWS FIGURE 2. ISTA audit feedback for 2014 36 audits thereof 20 feedbacks submitted. a higher response a proposal for improvement of the audit feedback form was discussed and the ISTA Accreditation Department will work on the new version. In 2014 out of 36 audits the ISTA Accreditation Department received 20 responses Figure 2. One audit was not positively rated and the corrective actions for this rating were implemented. Quality Assurance Workshop Plan Due to limited capacity no Quality Assurance Workshop was organized in 2015. Work has been started to facilitate a Quality Assurance Workshop in Estonia prior to the ISTA Congress 2016. Audit Checklists A change in the system checklist was presented. The current practice is that at the first audit auditors do not evaluate monitoring procedure and frequently at the very advanced audits no implementation of the monitoring procedure could be witnessed. The new agreement is to change this policy and to require an SOP of staff monitoring at the first audit and already a discussion of its future implementation. A finalized version of the common technical checklist is in use. The current practice was that each technical auditor has a personal technical checklist. A common technical checklist has been developed and technical auditors are welcome to contribute to its continuous improvement. Audit Findings Findings of the audits that took place from 1 July 2014 to 15 March 2015 were presented as follows substantial S and non-substantial NS non-conformities per related technical areacommittee Figure 3 system and technical non-conformities per related technical areacommittee Figure 4 categorization of system findings substantial S and non-substantial NS Figure 5. FIGURE 3. Substantial S and non-substantial NS non-conformities categorized per technical areacommittee. FINDINGS PER COMMITTEE SNS 01.07.2014 - 15.03.2015 FIGURE 4. System and technical non-conformities categorized per technical areacommittee. FINDINGS PER COMMITTEE SYSTEMTECHNICALBOTH 01.07.2014 - 15.03.2015 20 18 16 14 12 10 8 6 4 2 0 prep phase reporting CAevaluation Communication effective Evaluation criterias clear Audit relates to its purpose Conclusions relevant Audit was polite Lab view accepted Questions OK answered time management overall impression strongly agree agree partially agree disagree strongly disagree 40 35 30 25 20 15 10 5 0 BSC PUR GER MOI TZ VIG SH TSW GMO 29 audits thereof 3 GMO audits S NS 60 50 40 30 20 10 0 BSC PUR GER MOI TZ VIG SH TSW GMO 29 audits thereof 3 GMO audits System Technical both 15SEED TESTING INTERNATIONAL OCTOBER 2015 Technical Discussion The ISTA Accreditation Standard will be revised by the ISTA Executive Committee to clarify ISTA accreditation for sampling entities. The updated version shall be distributed to the Member Laboratories and published on the ISTA web site. It was discussed how to report the sealing of the seed lot on the Orange International Seed Sample Certificate. This was a subject for membership voting on the ISTA Rules Proposals 2016. A proposal for revising Annex 3 of the accreditation application form was discussed Table 2. It was decided to delete Crambe from crop group 1 grasses as it belongs to crop group 5 other agricultural species. Any moving of other species from group to group must be carefully considered because of the impact of any change within the crop groups on the accreditation and proficiency test records of the Member Laboratories. It may be possible to keep some species in two groups for example but in any case the Germination FIGURE 5. Categorization of the system findings substantial S and non-substantial NS. SYSTEM FINDINGS 01.07.2014 - 15.03.2015 TABLE 2. Annex 3 Crop groups for purity other seed determination and germination tests. Committee will be contacted for possible revision of the species grouping. A proposal has been made to include in either the ISTA Accreditation Standard or in the ISTA International Rules for Seed Testing a requirement for following the protocol for approval of the automatic samplers. The Bulking and Sampling Committee was contacted and the proposal for amending the ISTA Rules will be considered. The auditors suggested to create a portal on the ISTA web site or similar where audit topics that were discussed can be presented for future use of auditors. Crop group Size Species belonging to the following genera 1 grasses A Poa pratensis Poa trivialis Dactylis B Agrostis Anthoxanthum Crambe Cynodon Cynosurus Deschampsia Eragrostis Holcus Phleum Poa Schizachyrium C Agropyron Alopecurus Arrhenatherum Beckmannia Bromus Ehrharta Elymus Elytrigia Festuca XFestulolium Koeleria Lolium Pascopyrum Phalaris Piptatherum Psathyrostachys Pseudoroegneria Trisetum Zoysia D Andropogon Astrebla Bothriochloa Bouteloua Cenchrus Chloris Dichanthium Pennisetum Sorghastrum E Axonopus Brachiaria Digitaria Echinochloa Eleusine Melinis Panicum Paspalum Pennisetum glaucum Setaria Urochloa 2 cereals A Avena Hordeum Secale XTriticosecale Triticum B Oryza Sorghum Zea 3 small legumes A Aeschynomene Alysicarpus Anthyllis Astragalus Centrosema Chamaecrista Securigera Crotalaria Desmodium Galega Hedysarum Kummerowia Lespedeza Leucaena Lotus Macroptilium Macrotyloma Medicago Melilotus Onobrychis Ornithopus Securigera Trifolium Trigonella 4 pulses A Vicia small B Cajanus Cicer Lathyrus Lens Mucuna Phaseolus coccineus Pisum Psophocarpus Vicia large C Arachis Cyamopsis Glycine Lablab Lupinus Phaseolus Pueraria Vigna 5 other agricultural species A Beta Brassica Dichondra Hibiscus Linum Plantago Sinapis Spergula Raphanus B Cannabis Carthamus Fagopyrum Helianthus 6 vegetables including fruits spices and condiments A Achillea Anethum Anthriscus Apium Arctium Atriplex Atropa Camelina Campanula Carum Chrysanthemum Glebionis Cichorium Claytonia Cuminum Daucus Eruca Fragaria Lactuca Lepidium Solanum Solanum hybrids Marrubium Matricaria Melissa Mentha Nasturtium Nicotiana Ocimum Oenothera Origanum Papaver Petroselinum Phacelia Physalis Pimpinella Portulaca Rheum Rosmarinus Rumex Satureja Sesamum Stylosanthes Taraxacum Thymus Valerianella B Allium Asparagus Capsicum Corchorus Coriandrum Cynara Foeniculum Pastinaca Raphanus Sanguisorba Scorzonera Solanum Spinacia Tragopogon C Abelmoschus Borago Cucumis Cucurbita Cucurbita hybrids Citrullus Gossypium Ipomoea Lagenaria Luffa Momordica Ricinus Tetragonia 7 forest species See Table 2A Part 2 8 flower species See Table 2A Part 3 S 50 45 40 35 30 25 20 15 10 5 0 Doc control forms and records NCW and CAPA Int Audits Purchase Training and monitoring Staff JD List signatures Mgmnt rew Certificates and subcontracting SoA NS 16 SEED TESTING INTERNATIONAL www.seedtest.org ASSOCIATION NEWS Changes to the International Rules For Seed Testing 2016 Edition ISTA Publications Unit ISTA Secretariat 8303 Bassersdorf Switzerland jonathan.taylorista.ch Jonathan Taylor THIS YEARS RULES voting session at the Ordinary General Meeting was notable in that for the first time the electronic voting system with keypads was used successfully. Editorial Changes For this years editorial changes all cross- references within the Rules to other chapters and sections were systematically checked and any errors were corrected. Rules Changes and New Methods At the session of the Rules Committee on the previous day quite a number of proposals were amended. However with regard to the proposed change to Chapter 10 no consensus could be reached and the proposal was withdrawn. Thus the changes to the ISTA Rules for 2015 are as follows. Chapter 1 Certificates Clarification about issuance of certificates and other details related to duplicate and provisional ISTA Certificates 1.2 1.3 1.4 Clarification what should be entered under Seal of lot 1.4.2 amended proposal Chapter 2 Sampling Changes in 2.8 Tables for lot size and sample sizes Removal of herbage where differentiation between herbage and amenity turf is no longer appropriate 2.5.4.1 17.6 17.7 Chapter 3 The purity analysis Revision to Pure Seed Definition 4 re Helianthus seeds with fused pericarps Revision to 3.5.2 on retention of separated components other seeds only Chapter 4 Determination of other seeds by number Amendment of definitions for Other seeds determinations 4.2 4.5.1 Chapter 5 The germination test Germination tests allowed with only 200 seeds but only for Blue International Seed Sample Certificates 5.6.1 Clarifications on required actions when counting errors occur 5.6.1 Clarification on reporting germination when disinfection is applied 5.6.3.4 amended proposal Omitting the first count when germination tests are carried out in Organic Growing Media 5.6.4 Amendment to the process for retesting 5.7 5.9 amended proposal Chapter 7 Seed health testing Correction to text in existing seed health method 7-007 Modification to existing seed health method 7-022 with replacement of images Additional grow-out method for existing seed health method 7-026 Replacement andor addition of photographs in seed health testing methods 7-001a b 7-004 7-010 7-011 7-012 Chapter 9 Moisture determination Changes to methods for cutting seeds 9.1.5.2 9.1.5.5 Changes to methods for cutting large tree seeds for moisture testing 9.1.5.5 Chapter 11 Testing coated seeds Clarification of procedures for seed pellets 11.5 amended proposal 17SEED TESTING INTERNATIONAL OCTOBER 2015 17SEED TESTING INTERNATIONAL OCTOBER 2015 17SEED TESTING INTERNATIONAL OCTOBER 2015 17 TALLINN THE CITY WHERE MEDIEVAL MEETS MODERN WELCOMES DELEGATES TO THE ISTA 2016 CONGRESS 18 SEED TESTING INTERNATIONAL 2015 www.seedtest.org LADIES AND GENTLEMEN the 31st International Seed Testing Association Congress will be held from 15-21 June 2016 in Tallinn Estonia. The Ministry of Rural Affairs of the Republic of Estonia is delighted to be hosting the next ISTA Congress and would like to cordially invite you to Estonia. The Congress will take place in Tallinn a modern city in medieval clothing that was first marked on a map in 1154. The citys historic Old Town is included on the UNESCO World Heritage List. Following the Congress participants will also have the opportunity to discover other areas of Estonia. Two post-Congress tours to west and south Estonia are scheduled for 22-23 June 2016. The Congress is a unique opportunity for seed science specialists and industry representatives to meet and exchange work-related information and experiences. The event also provides an opportunity to present scientific discoveries and technical innovations as well as their practical applications. Moreover companies active in the field of seed science have the chance to promote their work in the exhibition area. We look forward to welcoming you in June 2016 to a highly professional successful and enjoyable event in wonderful Tallinn. Welcome to Estonia. Urmas Kruuse Minister of Rural Affairs Preliminary Programme Pre-Congress Workshops In conjunction with the 31st ISTA Congress 2016 two pre-Congress workshops for seed analyst training are scheduled from 10-13 June 2016. Germination Workshop The aim of the Germination Workshop is to present and discuss the principles of the ISTA germination test seedling evaluations for various species calculation and reporting of results and aspects of quality management in connection with germination testing. There is a minimum of 16 and a maximum of 20 participants. The workshop will take place in the Seed Testing Laboratory of the Agricultural Research Centre ARC in Saku. ASSOCIATION NEWS Quality Assurance Workshop for Advanced Laboratories The aims of the Quality Assurance Workshop for Advanced Laboratories are to improve quality assurance in seed testing laboratories according to the ISTA Accreditation Standard and to provide theoretical and practical exercises for better learning. There is a minimum of 16 and a maximum of 20 participants. The workshop will take place at the Board of Agriculture in Saku. For detailed information please visit the Congress website www.ista-tallinn2016.ee. ISTA Seed Symposium The ISTA Seed Symposium titled Improv- ing seed quality and developing seed testing through science and technology will take place from 15-17 June. Reports on improve- ments and developments of seed testing through science and technology will be given in oral presentations and poster sessions. ESTONIA 31st ISTA Congress 2016 TallinnRepublicofEstonia15-21June2016 19SEED TESTING INTERNATIONAL OCTOBER 2015 PROGRAMME OVERVIEW PRE-CONGRESS WORKSHOPS 10Fri to 13Mon ISTA workshop on germination Technical Workshop ISTA workshop on quality assurance for advanced laboratories Technical Workshop to be confirmed ISTA CONGRESS 14Tue Welcome reception 15Wed Opening ceremony Seed symposium 16Thu Seed symposium 17Fri Seed symposium Official dinner 18Sat Presentations technical work 19Sun Presentations technical work 20Mon Presentations technical work discussion forum 21Tue Ordinary general meeting Post-Congress Tours Two post-Congress tours are scheduled for 22-23 June 2016 directly after the ISTA Congress. All tours will depart from Tallinn and are planned to go to west and south Estonia. Further information about the itineraries will be made available on the Congress website www.ista-tallinn2016.ee. Travel Information Information about accommodation travel passport visa et cetera can be found on the Congress website www.ista-tallinn2016.ee. The Venue Tallinn The event will be hosted at the Estonian Concert Hall. This neoclassical imposing building is a symbol of Estonias political and cultural power. No other hall in Estonia can compete with Estonia Concert Hall in combining history with flexibility and beauty with adaptability. Registration Fees The registration fees will be announced on the Congress website www.ista-tallinn2016.ee on 1 October 2015. Provision will be made for ISTA Members and non-members. Trade Exhibition We would like to invite all exhibitors interested in reaching a target group of up to 400 seed science professionals from laboratories and organizations worldwide. The trade exhibition will be located in the foyer of the Congress venue. Sponsors There are possibilities to sponsor the ISTA Congress 2016 with a variety of sponsoring packages to choose from. Information about these can be found on the Congress website www.ista-tallinn2016.ee. Organization The 31st ISTA Congress will be hosted by the Ministry of Rural Affairs of Estonia and the Estonian Agricultural Research Centre. A National Organising Committee chaired by the Deputy Secretary General for Food Safety Research and Development of the Ministry of Rural Affairs has been established to prepare the Congress. Further information can be found on the ISTA Congress 2016 website www.ista-tallinn2016.ee. Accommodation All prices include VAT Original Sokos Hotel Viru Standard double room for one 76 Standard double room 81 SUPERIOR double room for one 96 SUPERIOR double room 101 Radisson Blu Sky Hotel Single room 135 Double room 145 Savoy Boutique Hotell Single room 105 Double room 115 Hotel Palace SUPERIOR Single room 88 Double room 96 Solo Sokos Hotel Estoria Solo King double room for one 88 Solo King double room for two 98 20 SEED TESTING INTERNATIONAL 2015 www.seedtest.org ASSOCIATION NEWS Congress 2016 Preliminary Programme Tuesday June 14 12001900 Congress registration desk open 18302100 Congress welcome reception Wednesday June 15 8001700 Congress registration desk open 8301730 ISTA Seed Symposium day 1 830930 Opening Ceremony Chair of the National Organising Committee NOC Welcome by the Minister of Rural Affairs of the Republic of Estonia ISTA President 9301000 Seed industry in Estonia 10301100 ISTA Seed Symposium 2016 Progress in seed testing and seed quality improvement through science and technology. Symposium convenor Dr. Laura Bowden Seed Physiologist - Official Seed Testing Station for Scotland Science and Advice for Scottish Agriculture SASA 11001230 Session 1 Applications of germination and dormancy testing. Chair and lead speaker Fernando Silveira Federal University of Minas Gerais Brazil Topics advances in the understanding of seed germination and dormancy characterisation of germi- nation physiology of germination and dormancy seed development and maturation dormancy breaking treatments image analysis 14301700 Poster session Thursday June 16 08001630 Congress registration desk open 08301630 ISTA Seed Symposium day 2 08301130 Session 2 Advances in seed health testing for ensuring quality seed production and storage. Chair and lead speaker Gary Munkvold Iowa State University USA Topics seed treatments epidemiology novel methods for seed health testing emerging diseases phytosanitary issues 11301430 Poster session continued 14301630 Session 3 Environmental effects on seed quality Chair and lead speaker Ilse Kranner University of Innsbruck Austria Topics stress tolerance seed vigour effect of the environment on seed development and production organic seed production maternal effects epigenetics climate change Friday June 17 08001700 Congress registration desk open 08301700 ISTA Seed Symposium day 3 08300930 Session 3 continued 09301230 Session 4 ISSS Collaborative Session Cellular and molecular methods and new approaches to seed quality determination. Chair and lead speaker Julia Buitink INRA France Topics genetics of seed quality QTL markers variety and GM identification new approaches to vigour and viability testing phenotyping imaging 13301630 Session 5 Conservation and use of genetic resources in crop forest and wild species. Chair and lead speaker Mati Koppel Estonian Crop Research Institute Estonia Topics collection of plant genetic resources seed storage and longevity desiccation tolerance plant breeding habitat restoration genetic resources in forestry 16301700 Overview of Symposium Conclusion of Symposium 19002300 Official Congress dinner and presentation of Seed Symposium awards RegistrationFeesTimelines The registration fee will be announced on the website of the 31st ISTA Congress www.ista-tallinn2016.ee on 1 October 2015. Registration will be possible from 1 October 2015 Early registration accepted until registration submitted and fee paid by that date 28 February 2016 Late registration closes on registration submitted and fee paid by that date 1 June 2016 21SEED TESTING INTERNATIONAL OCTOBER 2015 Saturday June 18 08001700 Congress registration desk open 09001800 Presentations of ISTAs technical work and meetings of ISTA Technical Committees - Bulking and Sampling Committee - Flower Seed Committee - Forest Tree Shrub Seed Committee - Germination Committee - Moisture Committee - Variety Committee - Statistics Committee - GMO Committee - Nomenclature Committee - SST Editorial Board Sunday June 19 08451730 Presentations of ISTAs technical work and meetings of ISTA Technical Committees continued - Purity Committee - Seed Health Committee - Seed Storage Committee - Tetrazolium Committee - Seed Vigour Committee - Advanced Technologies Committee - Rules Committee Monday June 20 08451030 Presentations of ISTAs technical work and meetings of ISTA Technical Committees continued Proficiency Test Committee Laboratory Accreditation and Quality Assurance Programme 1100-1630 Programme to be defined by National Organising Committee Tuesday June 21 08301730 ISTA Ordinary General Meeting Welcome by the ISTA President Jol Lchapp 1. Call to order 2. Presidents address 3. Roll call of Designated Members entitled to vote 4. Comments about the minutes of the previous General Meeting 5. Report of the Executive Committee 6. Report of the Secretary General 7. ISTA Strategy 8. Election of Officers and members-at-large of the Executive Committee 9. Fixation of annual subscriptions 10. Consideration and adoption of the proposed changes to the ISTA Articles 11. Consideration and adoption of the proposed Rules changes 12. Consideration and adoption of reports 13. Announcement of the place and date of the next Ordinary General Meetings 14. Any other business raised by a Member of which notice in writing has been received by the Secretary General at least two months prior to the date of the General Meeting 15. Any other business raised by consent of the Executive Committee 16. Discharge of the Executive Committee 17. Installation of new Officers and Executive Committee members 18. Presidents closing address 19. Adjournment 22 SEED TESTING INTERNATIONAL 2015 www.seedtest.org Estonia is proud of its traditional dark rye bread. The worlds oldest rye variety still being cultivated is Sangaste rye. The robust high-yielding long-strawed and frost-resistant variety was developed in 1875 in Estonia by count Freidrich Georg Magnus von Berg. Estonia clean and scenic. The purity of the countrys air is exemplary the water of its wild lakes is clear and a tranquil silence covers its sparsely inhabited countryside. Estonia a country the size of 45227 square kilometres is home to 1.3 million people making the average population density 34 people per km2 in some areas the average is even lower only 6.5 people per km2 . ASSOCIATION NEWS ESTONIA IS NORTH EUROPES BEST-KEPT SECRET. It is a small country with a rich inner life a young state long overshadowed by its older and better-known neighbours. Estonia a modern e-state where innovative ideas germinate. Estonia has the largest number of successful start-up companies per capita in the world and is the birthplace of such famous start-ups as Skype TransferWise and GrabCAD. Estonia is the first state to have built a charging network for electric cars that covers the whole country. In Estonia electronic services have become the norm e-elections e-taxes digital prescriptions and e-banking are just some of the e-services available. The advanced public and private e-services are available to citizens of other countries by applying for electronic residency through the e-Residency program. The Estonian Biobank at the Estonian Genome Center in Tartu is one of the worlds most representative population-based genetic data collections. To date the biobank has gathered health and genetic data from almost 52000 donors. Did You KnowInterestingFactsAboutEstonia Estonias wooded meadows are among the most species-rich biomes in the world up to 70 different plant species may grow on one square metre. The famous white nights. There are four seasons in Estonia and the average annual temperature is 5 C. The average temperature in July the warmest month of the year is about 20 C. The end of June is the time of white nights a time when the nights never get dark. The longest summer day lasts for 18 hours and 40 minutes from sunrise to sunset. The singing nation. The nations most important cultural event is the Song and Dance Celebration held in Tallinn every five years. The festival has been inscribed on the UNESCO Intangible Cultural Heritage List. The Song Celebration assembles one of the largest choirs in the world with up to 30000 singers typically taking part. Grannies on motorbikes. Only on the Estonian island of Kihnu can you see old ladies dressed in colourful folk costumes riding around on sidecar motorbikes. The unique way of life of the 600 inhabitants of Kihnu and Manija islands has been included in UNESCOs Intangible Cultural Heritage List. Photos by Kaupo Kalda Lembit Michelson Graham Mitchell Kristo Me Jaak Nilson Kaul Nurm Katrin Press Sven Zaek 23SEED TESTING INTERNATIONAL OCTOBER 2015 ESTONIA IS A GREEN COUNTRY. More than half of its territory is covered with forests half of the rest is covered with bogs and marshes agricultural land makes up about 20 per cent of the mainland. The countrys temperate climate and fertile fields have created good conditions for the development of agriculture. Estonia ranks first in the European Union in agricultural land area per person three quarters of a hectare per person. Estonian agriculture is characterised by the concentration of production into large holdings. The average size of agricultural Agriculture In Estonia holdings is about 50 hectares which is about four times more than the EU average. Crop cultivation as well as dairy and beef cattle farming are the most important sectors of agriculture in Estonia. Milk production traditionally holds the largest share in the value of the countrys agricultural production. Estonias strength lies in its grasslands and feed production. Grasslands cover roughly one third of the total agricultural land. 15 per cent of Estonias agricultural land is organic. This indicator ranks Estonia as third in the European Union. Ministry of Rural Affairs of the Republic of Estonia Lai tn 39 Lai tn 41 15056 Tallinn Estonia Phone 372 625 6101 Email pmagri.ee www.agri.ee Estonian Agricultural Research Centre Teaduse 46 Saku 75501 Harjumaa Estonia Phone 372 672 9137 Email infopmk.agri.ee httppmk.agri.ee 31st ISTA Congress Hostedby 24 SEED TESTING INTERNATIONAL www.seedtest.org ASSOCIATION NEWS Second Call for Papers 31stISTACongressSeedSymposiumTallinnEstonia15-17June2016 ESTONIA IS A SMALL COUNTRY with a low population. Despite its small size and population Estonia is a country rich in diversity natural resources and technology. There are a wide variety of natural habitats found in Estonia and a great amount of diversity. More than half of the country is covered with forests a further quarter with bogs and marshes with agriculture accounting for about 20 per cent of land use. The majority of Estonias forests are commercial however a significant proportion are now protected areas and Symposium Convenor Member ISTA Moisture Committee Member Seed Science and Technology Editorial Board Official Seed Testing Station for Scotland Science and Advice for Scottish Agriculture SASA Edinburgh EH12 9FJ UK Laura.Bowdensasa.gsi.gov.uk Dr. Laura Bowden nature reserves. The most common forest species are pine birch and spruce. Of the agricultural land cereals make up the largest crops with oilseeds and legumes increasing in importance more recently. There is also a lot of grassland and a significant proportion of organic farming. Both plant breeding and genetic resources are important in Estonia with the country becoming increasingly involved in restoration projects in recent years. Estonia in a country of innovation and has the largest number of start-up companies per capita in the world the most well- known of these being Skype. To reflect these technological advancements in the country the theme that has been chosen for the 31st ISTA Seed Symposium is Progress in seed testing and seed quality improvement through science and technology. 25SEED TESTING INTERNATIONAL OCTOBER 2015 The Symposium will be made up of five oral sessions and two poster sessions. Session themes have been selected to encourage papers from a diverse range of seed testing and seed research areas as well as to reflect some of the topics that are particularly relevant to Estonia. The Symposium will bring together seed analysts technologists researchers and managers from universities research institutes government and the seed trade to discuss all aspects of seed quality. The Symposium will provide a forum for the discussion of recent advances in seed science and technology as well as an opportunity for the exchange of ideas and information. At intending participants are encouraged to submit oral and poster papers topics under the above theme. The research reported in offered papers can cover both the scientific basis of aspects of seed quality and its technological application in seed testing. In all sessions we welcome papers on established and novel seed testing methods and on tropical and temperate crop species wild species flowers trees and shrubs including species with potential for use in plant breeding and in habitat regeneration. A book of abstracts containing invited and selected oral and poster presentations will be available to all Symposium participants. Session Topics Session 1 Applications of Germination and Dormancy Testing Lead Speaker Fernando Silveira Federal University of Minas Gerais Brazil Title Using tools from ecology and evolution to maximize the use of native seeds in species conservation and habitat restoration Topics Advances in the understanding of seed germination and dormancy Characterisation of germination Physiology of germination and dormancy Seed development and maturation Dormancy breaking treatments Image analysis Session 2 Advances in Seed Health Testing For Ensuring Quality During Seed Production And Storage Lead Speaker Gary Munkvold Iowa State University United States of America Title Emerging disease issues affecting international seed movement and seed health test development Topics Seed treatments Epidemiology Novel methods for seed health testing Emerging diseases Phytosanitary issues Session 3 Environmental Effects on Seed Quality Lead Speaker Ilse Kranner University of Innsbruck Austria Title Effects of the maternal environment on seed quality Topics Stress tolerance Seed vigour Effect of the environment on seed development and production Organic seed production Maternal effects Epigenetics Climate change Session 4 ISSS Collaborative Session Cellular and Molecular Methods and New Approaches to Seed Quality Determination Lead Speaker Julia Buitink INRA France Title A systems biology approach to understand and predict seed quality Topics Genetics of seed quality QTL markers Variety and GM identification New approaches to vigour and viability testing Phenotyping Imaging Session 5 Conservation and Use of Genetic Resources in Crop Forest and Wild Species Lead Speaker Mati Koppel Estonian Crop Research Institute Estonia Title Conservation and use of genetic resources in crop forest and wild species in Estonia Topics Collection of plant genetic resources Seed storage and longevity Desiccation tolerance Plant breeding Habitat restoration Genetic resources in forestry Submission of Papers Offers of papers should be submitted online only httpwww.seedtest.orgen abstractpaper-submission.html in the form of an abstract in English of 400 words maximum. Papers will be presented orally and in poster form both forms having equal status. Papers will be selected for presentation by the Symposium Convenor and a small scientific committee. As the number of oral presentations will be limited by time constraints oral presentation of your paper may not be possible and you may be asked to present your paper as a poster. Timeline 23 October 2015 Deadline for submission of all papers. Authors of papers considered for oral presentation will be contacted for further information on experimental results additional to the abstract. 18 December 2015 Authors informed whether papers have been accepted for oral or poster presentation. Authors of papers not accepted for oral presentation may be invited to present their work as a poster. 12 February 2016 Deadline for payment of registration fee by authors of accepted oral papers. If the presenter of an oral paper has not registered the paper will be replaced in the programme 11 March 2016 Deadline for payment of registration fee by authors of accepted poster papers. If none of the poster authors has registered by this time it will not be possible to present the poster and the abstract will not be published. Funding Authors of proposed papers are encouraged to explore possible sources of funding for their attendance at the Symposium as early as possible. ISTA cannot offer any financial support to authors of papers. However a letter of acceptance of a paper for presentation can be provided to assist in funding applications from early January 2016. 26 SEED TESTING INTERNATIONAL www.seedtest.org ASSOCIATION NEWS Successful Start for ISTA on Social Media Head of Marketing ISTA Secretariat 8303 Bassersdorf Switzerland pierrick.marcouxista.ch Pierrick Marcoux IN APRIL 2015 ISTA made a first significant step into the world of social media it published its very first post on LinkedIn the leading platform for professional networking. This new tool is one amongst many others to come for promoting the Association and raising its profile within the seed world. Unlike other social media platforms LinkedIn is strictly dedicated to professionals. It connects the worlds professionals to make them more productive and successful. When you join LinkedIn you get access to people jobs news updates and insights that help you be great at what you do. In the case of ISTA LinkedIn should be seen as a new and modern complementary tool to the existing tools ISTAs website newsletter and publications. On the ISTA LinkedIn page not only will you be able to get all the latest regulatory news previews of newly released publications and workshops and events announcements but also information on newly accredited laboratories articles about ISTA in various seed publications job opportunities in ISTA and much other useful information about the Association seed testing or the seed world in general. One important advantage of ISTA going digital is the ability to share updates with the community immediately enabling all stakeholders to remain informed in real time. By the time you read this article is written the ISTA page will have already reached 450 followers from all regions of the world and the community is growing daily Like share comment you can contribute Most importantly all of you can play a role in this new adventure. No word other than community can best describe what ISTA really is. And there is no such powerful tool as LinkedIn to keep in touch with your professional community By FOLLOWING ISTA on LinkedIn not only you will join and belong to the ISTA community but you will also have the opportunity to contribute to your community. To give concrete examples LinkedIn allows you to SHARE any post. Use the SHARE button to send useful information to your supervisor colleagues partners By clicking the LIKE button you will demonstrate your support for this initiative or that announcement By COMMENTING a post you will voice your opinion. Anytime you decide to COMMENT you can start a discussion request feedback from the community or ask for help with a technical challenge Being active on ISTAs LinkedIn page is just another way of supporting ISTAs vision of uniformity in seed testing within the global seed community. To sum up with its company page on LinkedIn ISTA is providing its Members and non-members with a very powerful forum to acquire information exchange ideas and develop a professional network. ISTA is looking forward to meeting you on LinkedIn very soon. Stay connected How to Find the ISTA Page on LinkedIn Once you have an account on LinkedIn go to LinkedIn www.LinkedIn.com and then enter ISTA in the search bar. ISTA appears at the bottom of the list Click the FOLLOW button Thats it You will now automatically receive all updates from ISTA. Can I submit content to be posted on the ISTA page on LinkedIn YES ISTA encourages all ISTA enthusiasts to submit content. Examples research findings event announcements new developments in technology Any topic that supports uniformity in seed testing and can benefit the ISTA community will be considered for posting. Please submit your content suggestions to pierrick.marcouxista.ch Already 450 followers of the ISTA page on LinkedIn are you one of them Keep in touch with the seed testing community follow ISTA on 27SEED TESTING INTERNATIONAL OCTOBER 2015 The ISTA Hop Hornbeam in the State Arboretum in Stuttgart-Hohenheim DidyouknowthatISTAistheproudholderofatreeThetree standsintheStateArboretumoftheHohenheimGardensatthe UniversityofHohenheimStuttgartGermany.Howcome TEN YEARS AGO as a long-standing member of the Statistics Committee Prof. Dr. Michael Kruse hosted the 7th ISTA Seminar on Statistics at the University of Hohenheim from 29 August to 2 September 2005. Sunny weather with blue skies contributed to a wonderful stay which involved not only statistics but also walks around Hohenheim and trips to the surrounding areas the state capital of Stuttgart the medieval town of Esslingen and the landscape of the Swabian Alb with caves castles and the Hohenheim Experimental Station for Plant Breeding. The Statistics Committee Vice-Chair Kirk Remund reported briefly in Seed Testing International No. 131 p. 41 April 2006 showing a photograph of 29 of the 32 participants. In addition a CD was produced containing the 32 presentations and 88 photographs of the seminar work and the tours. During the stay ISTA alumnus Adolf Martin Steiner formerly Custodian of the University Gardens gave a guided tour through the famous State Arboretum. The visitors were most impressed by the variety and scientific value of the collection of woody plants in a scenic area of more than 16 hectares part of the Hohenheim Gardens. The Arboretum comprises a total of approximately 1050 species and 1400 garden varieties the oldest having been planted in 1779 by Duke Charles 1 ISTA Alumnus 2 ISTA Personal Member University of Hohenheim Hohenheim Germany am.steineruni-hohenheim.de michael.kruseuni-hohenheim.de A. M. Steiner1 and M. Kruse2 Eugene of Wrttemberg whose summer residence was Hohenheim Castle. Eventually the spontaneous idea was advanced to sponsor a tree by which to remember the Seminar. After joint consultation a young Hop Hornbeam Ostrya carpinifolia Scop. was chosen which had been planted only a year before. This tree species is not indigenous to Germany but occurs in the Northern Mediterranean from Southern France to Turkey. It was a risk to choose such a young tree that had only recently been transplanted but it grew on a prominent site. To its south stands a Fagus sylvatica Purpurea Latifolia a National Champion Tree of Germany. To the north is a Carya cordiformis K. Koch a State Champion Tree of Baden-Wrttemberg. To the west stands a common oak Quercus robur L. and to the east lies the much-used central walk image at left. The choice was a full success. Today after 10 years the tree is nearly 10 metres high and its diameter at chest height is 12 cm. Hop Hornbeams have a hard wood and are often called ironwood. They are fast growing and can reach a height of more than 20 metres a diameter of up to 1.50 metres and an age of more than 100 years. The ISTA Hop Hornbeam is healthy and strong and looks forward to a promising future. It may be considered as a token for ISTAs future and in particular for that of the ISTA Statistics Committee. Come and pay a visit you are heartily welcome. 28 SEED TESTING INTERNATIONAL www.seedtest.org ASSOCIATION NEWS ISTAs First Industry Members Dow AgroSciences and DuPont Pioneer THE INTERNATIONAL SEED TESTING ASSOCIATION ISTA announces Dow AgroSciences and DuPont Pioneer as its first Industry Members. The recently introduced category of Industry Member aims to further engage the seed industry streamline communication and in the process expand the Associations activities. It is an honour to see that two of the main pillars of the seed industry are committed to support the Association says Beni Kaufman ISTAs Secretary General. As the name reflects this category is meant to all members of the industry large corporations small companies and commercial laboratories alike adds Kaufman. From the seed company point of view a new and important benefit in taking on the Industry Membership is partaking in a new body the Industry Advisory Group. Head of Marketing ISTA Secretariat 8303 Basserdorf Switzerland pierrick.marcouxista.ch Pierrick Marcoux This forum will be made of up to 15 ISTA Members and will be charged with the task of representing the industrys interest in the Association. At DuPont Pioneer we see this new membership category as an opportunity to move international seed trade testing toward standardisation whether physiological genetic and trait purity or physical properties which is important to maintain the integrity of the global seed trade says Jeremy Bergstrom DuPont Pioneer Global Quality Testing Manager. Collaboration is very important to Dow AgroSciences and this forum is a way to partner with the association and industry to focus on the seed quality needed by customers says Janice Hotz Director of Quality Dow AgroSciences. Improved technical testing methods will benefit farmers as they do the essential work of feeding a growing population. One seat on the Industry Advisory Group is reserved for a representative of the International Seed Federation ISF and the first eight Industry Members will automatically have a place on the group after ISTA executive committee approval. I believe further industry collaboration communication using these industry memberships as a platform help influence seed testing rules and expedite the acceptance of newer more precise seed testing technologies declares Bergstrom. The ability of ISTA to promote uniformity and standardisation in seed testing is a function of ISTAs recognition. Engaging as an Industry Member is an endorsement showing the industrys support to the Association and what it stands for. 29SEED TESTING INTERNATIONAL OCTOBER 2015 New ISTA Members Status 12 March 201514 August 2015 Industry Members Laboratory Members CONGRATULATIONS ON BECOMING ISTA MEMBERS United States of America DuPont Pioneer 7100 NW 62nd Avenue Johnston IA 50131 Dow AgroSciences Bldg 312 9330 Zionsville Road Indianapolis 46268 Burkina Faso BFML0100BRML0101 Laboratory representative Mrs. Esther Kargougou SOFITEX Physiology and Genetic Puri- ty Laboratory Seed Quality Control BP147 Bobo Dioulasso Phone 226 76 10 80 80 kargougou.esthersofitex.bf Egypt EGML0300EGML0301 Laboratory representative Prof. Dr. Yasser M. Shabana Mansoura University Tissue and Seed Pathology Laboratory Faculty of Agriculture Plant Pathology 60 El-Gomhoria Street El-Mansoura 35516 Phone 2 01149892220 yassershabana2yahoo.com Personal Members Associate Members Chile CLPM0005 Ms. Alejandra Ros Lab Oficial de Analisis de Semillas Servicio Agricola y Ganadero Km.22 Ruta 68 Cas. 4088 Santiago Phone 56 2 2345 1833 Fax 56 2 2345 1802 Alejandra.Rossag.gob.cl United Kingdom GBPM0015 Mrs.Heather Webb Germains Seed Technology Quality Assurance Hansa Road Hardwick Industrial Estate Kings Lynn PE30 4LG Phone 44 1553 774012 Fax 44 1553 773 145 hwebbgermains.com Uruguay UYDM0002 Dr. Vanessa Sosa Instituto Nacional de Semillas INASE Laboratorio Camino Bertolotti sn y Ruta 8 Km 29 Barros Blancos Canelones 91001 Phone 598 2 2887099 Fax 598 2 2887077 vsosainase.org.uy Nigeria NGAM0002 Dr. AWO Marie Ayodele International Institute of Tropical Agriculture IITA Oyo Road Ibadan PMB 5320 Phone 234 8034 989 117 m.ayodelecgiar.org Poland PLAM0001 Mr. Mateusz Gramza BIOTEK Agriculture Polska Sp. Z o.o. Gac 64 55-200 Olawa Phone 71 301 44 62 Fax 71 301 44 62 gramzabiotek-agriculture.pl NEW MEMBER COUNTRY Become a Member of ISTA TodayThe ISTA membership is a collaboration of seed scientists and seed analysts from universities research centres and governmental private and company seed testing laboratories around the world. ISTA values and promotes the diversity of membership this being the basis for its independence from economic and political influence.The ISTA membership consists of Member Laboratories Personal Members Associate and Corporate Members. To learn more visit www.seedtest.org. Membership Benefits Include Receive the ISTA Rules - Get valuable information through ISTA publications - Be prioritised in any ISTA event - Be involved in the seed testing methodology development - Participate in the ISTA Proficiency Test Programme - Become accredited and issue ISTA International Certificates - Participate in the ISTA decision making process 29SEED TESTING INTERNATIONAL OCTOBER 2015 30 SEED TESTING INTERNATIONAL www.seedtest.org RULES DEVELOPMENT Validation Study for the Germination of Dactylis glomerata and Festuca arundinacea Innoseeds BV 4421 AJ Kapelle Netherlands harry.nijensteininnoseeds.nl margriet.vaneekeleninnoseeds.nl J. Harry Nijnstein and Margriet van Eekelen Summary A validation study on the duration of the germination test for Festuca arundinacea and Dactylis glomerata was carried out. Eight ISTA-accredited laboratories in seven countries on three continents participated. Four samples per species were germinated at 1525C and 2030C. Seedling counts were made after 7 10 and 14 days in F. arundinacea and after 7 14 and 21 days in D. glomerata. Differences in germination between 21 and 14 days in D. glomerata were on average 2.7 and 2.9 for low and high temperatures respectively. In F. arundinacea these differences were 6.6 and 3.2 respectively. Individual samples differed up to 11.6 in F. arundinacea and up to 5.1 in D. glomerata. Repeatability and reproducibility were similar for the last two counts in D. glomerata and were at acceptable levels. In F. arundinacea only reproducibility at the final count after 14 days was at an acceptable level second count reproducibility was not at an acceptable level. Repeatability for F. arundinacea was at an acceptable level. Because of the sometimes large differences between the two last counts in both species it is suggested to keep the current duration of the germination tests of D. glomerata and F. arundinacea in the ISTA Rules. Introduction The durations of the germination tests for Festuca arundinacea tall fescue and Dactylis glomerata cocksfoot orchardgrass are 14 and 21 days respectively. The prechill period if applied must be added to these periods. Shortening the duration will make the test more cost effective in the laboratory and will result in seed lots being available faster for shipping to customers. The ISTA Rules state that there should be for F. arundinacea a first count at 7 days and a final count at 14 days and for D. glomerata a first count at 14 days and a final count at 21 days. The Rules allow for a shortening of the duration of the tests under some circumstances. Our objective in these comparative tests was to see whether the test could be shortened for routine use from 14 to 7 days for F. arundinacea and from 21 to 14 days for D. glomerata. Previous work has shown that the genetic speed of germination has little or no effect on differences between the last two counts for Lolium perenne Festuca rubra and Poa pratensis Nijnstein 2013 tables 1 and 3. Therefore this aspect was not taken into consideration here. Materials and Methods Participating Laboratories Eight laboratories from seven countries in three continents participated in this validation study. All participating labs are ISTA accredited for germination of the grasses in test. Samples were coded Table 1 column 6 shipped on 24 July 2014 and received by the labs within one week. All tests were completed by mid-October. In the report labs are coded and in a order different from the above. Materials Seeds of F. arundinacea and D. glomerata were used in this study. Four samples per species were germinated including four different varieties for F. arundinacea and three for D. glomerata that varied in germination level Table 1. Samples covered three production years and two production locations countries. Germplasm is widely exchanged over the world both for breeding purposes new varieties contain genetic material from all over the world and for seed production of existing varieties. For example seed of varieties from Europe is widely produced in Northern America and New Zealand. In this validation study labs from all major seed production areas of these species were involved. These participating labs all have wide experience in testing all these different varieties. Therefore it is concluded that these varieties represent the number of worldwide occurring varieties. Seed lots free of other seeds were used. In order to avoid any influence of the purity test in individual labs seeds of all species were purified by the organising lab before dispatch. Purified means that the seeds were blown in order to remove all empty seeds. Other crop or weed seeds that may have been found occasionally were discarded and replaced by a pure seed in the germination test by the participating laboratory. Stanisavljevic et al 2011 reported that in D. glomerata dormancy may be present up to one year after harvest. Therefore in this species samples of different harvest years were selected. However because of the delay between the selection of the samples and the time of testing them in the participating labs even the youngest samples were germinated more than one year after harvest. 31SEED TESTING INTERNATIONAL OCTOBER 2015 Table 1 shows the characteristics per sample at the time of selection. Test Method If not indicated otherwise labs adhered to the instructions as in the ISTA Rules ISTA 2013. Germination temperatures may affect the speed of germination. Therefore samples were germinated at all temperatures that are allowed according to the ISTA Rules ISTA 2013. For both species these were 15 25 and 20 30C. Dormancy breaking is optional in the ISTA Rules. For grasses almost all labs simultaneously apply all three dormancy- breaking methods allowed prechill alternating temperatures and KNO3 . As the number of days of prechilling will influence the results of the later counts in this study prechill was set at two days at 7C. As many laboratories apply a 57 day prechill period we assumed to be on the safe side. In F. arundinacea counts were carried out after 7 10 and 14 days thus testing the hypothesis that no extra normal seedlings would appear after day 10. In D. glomerata counts were made after 7 14 and 21 days assuming no extra normal seedlings appearing after day 14. See table 2 for details on tests and test conditions. Analysts were asked to consider the intermediate 10-day count in F. arundinacea and the intermediate 14-day count in D. glomerata as final questionable seedlings or in doubts were to be classified. Also abnormal seedlings were registered but not to be taken from the blotters in this intermediate second count. Labs were asked to report normal seedlings abnormal seedlings fresh non-germinated seeds and dead seeds. Two labs did not report fresh non-germinated seeds separately. Therefore fresh non-germinated seeds and dead seeds were added up into one category. The data received from the participants were checked for completeness and plausibility and found to be satisfactory. In order to limit the workload only normal seedlings will be dealt with in this report. This comparative test thus involved a total of 2 species 4 samplesspecies 2 temperaturessample 16 germination tests completed by each of the participants. Lab no. 3 was not able to do the 15 25 temperature regime. Therefore its results were not included in the analysis. Statistical analysis of the test results were performed by the ISTA Statistics Committee using methods derived from Generalized Linear modelling and the ISTA Tolerance approach and was similar to the analysis described in Nijnstein 2013. Results Comparison of Species Temperatures and Durations of the Test In F. arundinacea the average difference between the last two counts is much larger at 15 25 C 6.6 than at 20 30C 3.2. At both temperatures lot 4 shows the largest difference between the last two counts. In D. glomerata these differences were 2.7 and 2.9 at low and high temperature respectively. Differences per individual sample vary from 0.4 to 11.6 in F. arundinacea and from 0.1 to 5.1 in D. glomerata Table 3. Temperature effects at the final count are small in both species 80.2 and 80.8 in F. arundinacea and 91.5 and 91.4 in D. glomerata for low and high temperatures respectively. At the second count differences in D. glomerata are still small 88.8 and 88.5 but larger in F. arundinacea at 73.6 and 77.6 Table 3. Species Variety Sample id company Harvest year Harvest country Sample id ISTA study First count 7 d Germination 1421 d Sample date F. arundinacea Greenfront 344576 2011 NL Fa1 64 92 2013-12-19 F. arundinacea Rhizing star 364025 2012 NL Fa2 49 86 2013-04-18 F. arundinacea Hidalgo F0111Z213855A03 2012 F Fa3 96 97 2013-07-01 F. arundinacea Exella 3304010 2013 NL Fa4 73 83 2013-07-26 D. glomerata Pizza 244914 Before 2007 NL D1 61 79 2013-12-19 D. glomerata Amba DK-12DF0283 2011 DK D2 85 93 2012-11-28 D. glomerata Trerano DK-13BA1056 2012 DK D3 93 93 2013-02-04 D. glomerata Amba DK-14BA1218 2013 DK D4 93 94 2013-09-25 TABLE 1. Characteristics per sample Species Substrate Temperature C First count days Second count days Final count days Prechill days C Dactylis glomerata TP 20 30 15 25 7 14 21 2 d 7 C Festuca arundinacea TP 20 30 15 25 7 10 14 2 d 7 C TABLE 2. Overview of the tests and test conditions in this validation study 32 SEED TESTING INTERNATIONAL www.seedtest.org RULES DEVELOPMENT Initially all samples germinated at 79 or higher Table 1. In the study samples 2 and 4 of F. arundinacea germinated at 74 and 63 respectively Table 3. These two figures are below the minimum accepted in the market. This decrease was due to prolonged storage between selection of the samples and dispatch. In both species two out of four lots resulted in a statistically significant difference between the second and the final count Table 4. Further analysis revealed statistically significant effects for the following factors lot days counts and the interaction lot days indicating that not all samples responded in the same way results not shown. Averages and P values in red indicate statistically significant differences between the two values at p 1 one speaks of overdispersion because the data have a larger variance than expected under the assumption of a binomial distribution. See Nijnstein 2013 for details of computation. F. arundinacea Temperature C 15 25 15 25 15 25 20 30 20 30 20 30 Count days 7 10 14 7 10 14 Lot 1 39.9 81.7 90.3 66.3 86.4 90.6 Lot 2 50.4 70.3 74.7 63.7 72.1 74.4 Lot 3 80.0 93.0 94.9 91.6 95.1 95.6 Lot 4 21.0 49.3 60.9 37.9 56.7 62.6 Overall mean 47.8 73.6 80.2 64.9 77.6 80.8 Mean difference 1410 days 6.6 3.2 TABLE 3. Germination percentages at three counts at two temperature regimes D. glomerata Temperature C 15 25 15 25 15 25 20 30 20 30 20 30 Count days 7 14 21 7 14 21 Lot 1 36.7 81.3 86.1 50.7 83.1 87.0 Lot 2 52.0 88.4 90.3 94.6 88.6 90.7 Lot 3 92.1 94.7 94.9 91.7 93.7 94.3 Lot 4 54.0 90.9 94.7 59.1 88.6 93.7 Overall mean 58.7 88.8 91.5 66.5 88.5 91.4 Mean difference 2114 days 2.7 2.9 F. arundinacea 10 days 14 days P-value Lot 1 84.6 90.4 33SEED TESTING INTERNATIONAL OCTOBER 2015 In F. arundinacea there was no overdispersion for temperatures 15 25 at 14 days but there is an overdispersion in all five other situations. fR values are smaller than the f values for temperatures 20 30 but not for temperatures 15 25 the fR values are smaller than the f values at 14 days for the two temperatures. At the 7-day count the fR values are much higher than f. At the 14 and 21 days counts the fR values are still higher than the fR values for both temperature regimes. In both F. arundinacea and D. glomerata fR values are biggest at the first count and smallest at the final counts. Discussion For D. glomerata there were no differences between the two temperature regimes at 14 and 21 days. In F. arundinacea the difference was small after 14 days but 4.0 after 10 days Table 3. In the previous study these values varied between 0.7 and 2.9 Nijnstein 2013 Table 2. Higher temperatures may speed up the germination process. This was shown in F. rubra and P. pratensis first counts in F. rubra were considerably higher after 7 days at 20 30C 57.9 than at 15 25C 45.1. In P. pratensis first counts at 20 30C 56.2 were higher compared to 15 25C 46.0 and 10 30C 16.6 Nijnstein 2013. This suggests that in some grasses the highest temperature allowed in the ISTA Rules should be applied for the fastest finalization of germination. The decrease in percentage germination for samples 2 and 4 of F. arundinacea germinating only 74 and 63 respectively may have influenced the speed of germination negatively especially for sample 4. Lower quality often results in slower germination. This does not explain the differences between the last two counts in sample 1 of this species however as this sample germinated 90. Differences between the last two counts for D. glomerata were relatively small and at the same level as for F. rubra in the previous study Nijnstein 2013. The maximum differences for individual samples in F. rubra 5.8 were similar to those in D. glomerata in this study 5.1. However in the present study four larger differences in D. glomerata 3.9 3.9 4.9 5.1 were found compared to the only one big difference of 5.8 in F. rubra in the previous study. Repeatability in this study showed some overdispersion at a level similar to the level observed in the previous study Nijnstein 2013. This is acceptable as the overdisperion was only slight and similar for all combinations of temperature and duration tested including the final count as it is in the present ISTA Rules. Reproducibility was relatively good at 10 days 15 25 for F. arundinacea. All other combinations tested were at an acceptable level. For D. glomerata reproducibility was just out of tolerance for all RESULTS OF REPEATABILITY FR FOR F. ARUNDINACEA Temp. Normal 7 days Normal 10 days Normal 14 days 15 25 1.20 1.08 0.92 20 30 1.22 1.20 1.13 RESULTS OF REPEATABILITY FR FOR D. GLOMERATA Temp. Normal 7 days Normal 14 days Normal 21 days 15 25 1.26 1.09 1.13 20 30 1.08 1.29 1.03 In Dactylis there is overdispersion in all situations. Statistical Analysis Calculation of Reproducibility Reproducibility is the precision under reproducibility conditions i.e. conditions where test results are obtained with the same method on identical test items in different laboratories with different operators using different equipment. fr 2 should be smaller than the f value defined by Miles 1963 in equation AG4 and which is used to develop ISTA tolerance tables for comparing germination results from different labs. See Nijnstein 2013 for details of computation. At the 7 days count the fR values are much bigger than f. At the 10 days count the RESULTS OF REPRODUCIBILITY FOR F. ARUNDINACEA Temperatures Normal 7 days Normal 7 days Normal 10 days Normal 10 days Normal 14 days Normal 14 days fR f fR f fR f 15 25 6.80 1.92 3.62 1.77 1.56 1.72 20 30 2.63 1.82 1.34 1.73 1.30 1.71 RESULTS OF REPRODUCIBILITY FOR D. GLOMERATA Temperatures Normal 7 days Normal 7 days Normal 10 days Normal 10 days Normal 14 days Normal 14 days fR f fR f fR f 15 25 6.32 1.89 2.27 1.64 1.90 1.62 20 30 6.95 1.82 1.99 1.64 1.73 1.62 34 SEED TESTING INTERNATIONAL www.seedtest.org RULES DEVELOPMENT combinations tested. As the differences were small and consistent results are at an acceptable level. Repeatability and reproducibility for D. glomerata in the present study were not as good as for F. rubra in the previous study. Repeatability of the second count was poorer for D. glomerata 1.14 than for F. rubra 0.95. Reproducibility of second count was poorer for D. glomerata 1.97 than for F. rubra 1.72. Conclusions In F. arundinacea it is questionable whether reproducibility is at an acceptable level. More importantly differences between the last two counting dates were sometimes considerable. The averages of the differences between the last two counts for D. glomerata are at an acceptable level. However the number of large outliers and the statistical evaluation lead to the conclusion that shortening of the duration is not acceptable. It is suggested to retain the current duration of the germination tests for F. arundinacea and D. glomerata. Acknowledgements The participating laboratories technical and statistical reviewers and Technical Committee Coordinator ISTA Secretariat are thanked for their contribution to this validation study. References Dodig D. 2010. Effects of the duration of after-ripening period on seed germination and seedling size in three fescue species. Spanish Journal of Agricultural Research 82 454-459. ISTA 2013. ISTA Rules 2013 International Seed Testing Association Bassersdorf Switzerland. Miles S.R. 1963 Handbook of tolerances and measures of precision for seed testing. Proc. Int. Seed Test. Ass. 28 3. Nijnstein J.H. 2013. Revised method and ISTA method validation report for the germination of Lolium x boucheanum L. multiflorum L. perenne Festuca filiformis F. heterophylla F. ovina F.rubra Poa nemoralis P. palustris P. pratensis. ISTA Method Validation Report. Method Validation Reports on Rules Proposals for the International Rules for Seed Testing 2014 Edition 2013 2-33. Stanisavljevic R. Djokic D. Milenkovic J. Dukanovic L. Stevovic V. Simic A. Dodig D. 2011. Seed germination and seedling vigour of Italian ryegrass cocksfoot and timothy following harvest and storage. Cienc. Agrotec Lavras 356 1141-1148. Where next ISTA is seeking Member Countries to host the Annual Meetings in 2018 and beyond. Applications are welcome from any ISTA Designated Authority of a Member Country. For more information about requirements application procedure etc. please see the guidelines available on the ISTA web site at www.seedtest.orghosting or contact the Secretariat at ista.officeista.ch 35SEED TESTING INTERNATIONAL OCTOBER 2015 Accreditation of the Seed Testing Laboratory FSBI Bryanskaya MVL in Russia Irina Burova The Seed Testing Laboratory FSBI Bryanskaya MVL 241520 Bryansk Russia bmvl32yandex.ru IN OCTOBER 2014 the seed testing laboratory FSBI Bryanskaya MVL located in Bryansk Russia obtained accreditation with the International Seed Testing Association. The laboratory was set up in 2007 with the aim of supporting activities carried out by the Federal Service for Veterinary and Phytosanitary Surveillance Rosselkhoznadzor in the Bryansk and Smolensk regions in the field of species and variety testing seed quality characteristics testing and evaluation of plant stock quality in compliance with the national standards under official seed control schemes. Since then the laboratory has increased the number and types of tests performed. It is constantly striving to expand and optimize existing capacity to meet customer expectations and improve a ACCREDITATION quality management system to keep pace with the times. Over a period of four years the analysts of laboratory FSBI Bryanskaya MVL gained the knowledge and practical experience to apply for accreditation with the National Seed Certification System Semstandard. In 2011 the laboratory became a Member of ISTA and launched the ISTA Accreditation programme. The activities undertaken by the laboratory staff included ISTA Proficiency Test Programme taking 2.5 years to achieve competency to the ISTA level. During this period 36 SEED TESTING INTERNATIONAL www.seedtest.org the analysts familiarized themselves with international seed testing policy and practice and implemented a quality management system to conform to the ISTA Accreditation Standard. ACCREDITATION Want to stay up-to-date with ISTA Follow them on for exclusive articles news and announcements The analysts attended ISTA workshops and the laboratory was extensively involved in both theoretical and practical training to enable the application of seed testing techniques covered by the ISTA Rules. The ISTA accreditation audit was conducted on 22 May 2014. The scope of accreditation for the laboratory FSBI Bryanskaya MVL includes seed sampling manual purity germination and viability test- ing other-seed determinations and moisture for cereals and other agricultural crops. The ISTA Accreditation of the seed testing laboratory is the result of teamwork. The laboratory proved its technical competence and the analysts could demonstrate their professional skills during the on-site assessment. The ISTA Accreditation opens up some completely new perspectives for the laboratory and outlines the key challenges for the future. We are very proud of this accomplishment and happy to be an ISTA Member Laboratory. 37SEED TESTING INTERNATIONAL OCTOBER 2015 ISTA Accreditation For Sampling Only Rita Zecchinelli ISTA Executive Committee Accreditation Working Group Consiglio per la Ricerca in Agricoltura e lAnalisi dellEconomia Agraria Sede di Tavazzano-Laboratorio 26838 Tavazzano LO Italy rita.zecchinellientecra.it THE ISTA EXECUTIVE COMMITTEE has approved a new ISTA Accreditation Standard version 6.0 effective from 1 August 2015. The new version of the Standard is now called ISTA Accreditation Standard for Seed Testing and Seed Sampling. It clarifies that ISTA is accrediting for all methods included in the ISTA Rules both for seed sampling and seed testing. The accreditation component represented by seed sampling is not new for ISTA. Most of the testing laboratories are accredited for different testing methods and for sampling as well and the previous version of the ISTA Accreditation Standard already offered the chance of ISTA accreditation for sampling only. Nevertheless this possibility did not seem to be well outlined and some definitions included in the standard could lead to confusion. This is the case of term laboratory previously defined as an entity performing seed sampling andor testing. Today a laboratory is an entity performing a seed testing and sampling or b seed testing only. The new version of the ISTA Accreditation Standard clearly states that the standard applies also to entities performing sampling only covering all steps from sampling to issuance of ISTA certificates. At the end of the document a revision history table has been introduced in order to facilitate the users identify the changes. The current ISTA Accreditation Standard for Seed Testing and Seed Sampling is available and can be downloaded from the ISTA website httpswww.seedtest.orgenrelated- documents-_content---1--1253.html. ISTA ACCREDITATION STANDARD FOR SEED TESTING AND SEED SAMPLING Introduction This standard specifies the criteria which must be fulfilled by laboratories in order to maintain their status as an ISTA accredited laboratory and their authorisation to issue ISTA certificates. This standard covers all steps from sampling to issuance of ISTA certificates. This standard applies also to entities performing sampling only. ISTA Certificates can be issued only by accredited laboratories having seed testing methods included in their scope of accreditation. Application forms are obtainable from the ISTA Secretariat. In order to obtain accreditation duly completed application forms must be lodged with the ISTA Secretariat. The applicant must meet the required organisational and other requirements outlined in this Standard show competence by successfully completing the pre-accreditation proficiency testing and demonstrate competence during the on-site assessment of the laboratorys facilities by auditors appointed by the ISTA Executive Committee. Applicants pay for the services rendered during the accreditation assessment proficiency assessment on-site assessment and document evaluation and pay an annual fee for being an accredited member of ISTA. ISTA accreditation is formally granted by ISTA after the Executive Committee has been satisfied that the accreditation process has been properly executed and that the applicant laboratory has met the requirements of this Standard. 38 SEED TESTING INTERNATIONAL www.seedtest.org THE ISTA EXECUTIVE COMMITTEE has approved another decision related to the revision of the ISTA Accreditation Standard. This decision installs a new ISTA Seed Sampling Certificate. As stated by the new version of the standard but in fact it was true even before the specified conditions apply to the laboratory and to the sampling entities as well. Therefore in order to be ISTA accredited an entity performing seed sampling only no testing methods are included in the Scope of Accreditation should fulfil among the others the requirement 9.2 of the ISTA Accreditation Standard Based on that should the sampling only entity be responsible to check the correctness of certificates issued by a laboratory for samples they have drawn If not how to fulfill the requirement of ensuring an audit trail The ISTA Seed Sampling Certificate Rita Zecchinelli ISTA Executive Committee Accreditation Working Group Consiglio per la Ricerca in Agricoltura e lAnalisi dellEconomia Agraria Sede di Tavazzano-Laboratorio 26838 Tavazzano LO Italy rita.zecchinellientecra.it The idea is that the entity which is ISTA Accredited for sampling could finalize its work completing a sampling certificate. This certificate will be provided to the seed testing laboratory strengthening the link between seed sampling and seed testing and will be kept by the sampling entity for traceability. The sampling certificate shall report a minimum set of information e.g. responsible for sampling seed lot identification and description. The sampling entity can add additional information if useful. During the last ISTA Ordinary General Meeting held on 18th June 2015 Montevideo Uruguay the ISTA Executive Committee presented to the membership the project to install an ISTA Seed Sampling Certificate. It means that ISTA will provide such a certificate to accredited sampling entities and laboratories to be completed with the relevant information some of them marked as mandatory others as optional. The use of the ISTA Seed Sampling Certificate will not be binding. The sampling entity will be free to use its own form provided that the mandatory information is recorded in that form. The use of such certificate will not be allowed when sampling and testing are performed by the same laboratory - in this case the ISTA Orange International Certificate only is issued. The discussion that followed the presentation at the meeting showed that the ISTA Seed Sampling Certificate might also have other uses. It could be useful when sampling is done by one accredited lab and testing by another one. It could prove to be convenient when ISTA accredited sampling entity wants to give evidence that sampling has been done using the ISTA Rules even if an ISTA certificate is not issued. The introduction of the new ISTA Seed Sampling Certificate up to next three years will be considered as an experiment a trial period. Latest in 2018 the ISTA Executive Committee will evaluate the success of the initiative and will ask for a vote by the membership. If it is positive the final introduction of the new ISTA Seed Sampling Certificate will be decided on together with the definition of the terms of use e.g. to continue with the use on a voluntary basis and the fee - until that vote the fee will be the same of the other ISTA certificates. The initiative to have a new ISTA Seed Sampling Certificate has been well received by the ISTA members. The practical experience will tell whether the certificate will provide a new tool useful for the ISTA community and stakeholders in addition to those already available. 9.2 The laboratory must maintain a document and a record system to suit its particular circumstances. It must retain all records of original observations calculations and derived data and sufficient information to derive an audit trail calibration records staff records and a copy of each test report issued for a defined period not less than six years. ACCREDITATION 39SEED TESTING INTERNATIONAL OCTOBER 2015 DuPont Pioneer Asia Pacific Regional Quality Lab ThefirstISTA-accreditedlaboratoryinthePhilippines Regional Quality Testing Manager for Asia-Pacific DuPont Pioneer Hi-Bred Philippines Luisita Industrial Park LIP San Miguel Tarlac Philippines greg.lozanopioneer.com Greg Lozano DUPONT PIONEERS ASIA Pacific Regional Laboratory also known as TARLAB made history when it became the first ISTA-accredited laboratory in the entire Philippines. TARLAB ISTA lab code PH03 gained its ISTA accreditation on 22 May 2015. The regional lab was inaugurated on February 18 2013 and started operating on March 1 2013. It is a fairly newly established laboratory actually the youngest of the regional seed quality laboratories in DuPont-Pioneer worldwide. We started our efforts for ISTA accreditation right away in 2014 with Lingyu Zhang. So it is a great achievement and a matter of pride to gain ISTA accreditation this year our second year in operation. The regional lab caters mainly to the seed quality testing needs of Pioneer in Asia Pacific including India Pakistan Indonesia Thailand Vietnam Philippines Australia New Zealand and Japan. They are our main internal customers. But aside from our own current Pioneer internal customers we will be providing or offering ISTA seed testing services to external customers. This year after having gained our initial accreditation we have an ambitious intention to extend our accreditation scope to several more types of tests for almost all crop groups. This will tremendously increase our capability to provide the widest possible array of testing options for multiple crops to as many external customers as possible. The success and strength of TARLAB lies in its people and strong core values on safety and health environmental stewardship highest ethical behavior and respect for people. We have a young energetic laboratory team led by lab leaders who are always buzzing with new and great ideas on how to achieve our goals. For us gaining ISTA accreditation is gaining a seal of proven world-class quality. We are very proud to be the first ISTA-accredited laboratory in the Philippines TARLAB is located at the Luisita Industrial Park in Tarlac City Philippines. The laboratorys nick- name TARLAB is a truncation of Tarlac the city where it is located. Florina Palada left and Joost van der Burg center auditing our sample receiving process for ISTA accreditation on 9 January 2015. The Asia Pacific Regional Lab Manager and Lab Leaders in their international costumes Left to right Daisy Soria QMR Alfred Real Lab Lead- er-Logistics Regulatory Compliance Sasang Abdusalam Lab Leader Germination Eval- uationSafety Health Environment SHE Co- ordinator Greg Lozano Manager Cha Realubit Lab Leader Molecular Testing Grain Quality and Emz Espejo Lab Leader Germination EvaluationSeed Treatment Safety Research Embryo Rescue 40 SEED TESTING INTERNATIONAL www.seedtest.org ACCREDITATION Results of GMO Proficiency Test 20 THESE ARE THE RESULTS AND RATINGS for the GMO Profiency Test PT 20. Experimental Design Samples were either negative i.e. did not contain any transgenic events or positive i.e. contained the transgenic events GHB614 andor LLCOTTON25. When the positive samples were prepared defined numbers of seeds were mixed with non-GM seeds. The purity of both seed lots used in this PT round GM and non-GM was checked by PCR and confirmed as meeting the purity standard required prior to sample preparation. Each participating laboratory received a sample set made up of eight numbered samples of cotton seed containing approximately 2000 seeds based on the thousand-seed weight. The sample sets comprised samples with spiking levels listed in table 1. Evaluation Sample sets were sent to 22 laboratories. 21 participants submitted their results 9 provided qualitative results only. Qualitative Rating The rating for the presence or absence qualitative results is based on a percentage of misclassified samples out of the total samples. Misclassification may be either a false positive or a false negative result. Missing results for individual samples are considered as misclassifications. Quantitative Rating The quantitative rating is based on the quantification results for the positive samples and their reference value samples with a zero spiking level are not used in the quantification rating. The reference value is either the number of GM seeds in percent the mass of the GM seeds in percent or the median of the results reported by the participants in the unit DNA copies. Which of the reference values is chosen is determined by the panel of experts appointed for each round the guiding principle being Spiking level 0 0.4 0.8 0.4 Event None GHB614 LLCOTTON25 GHB614 LLCOTTON25 Lot numbers 1 2 3 4 5 6 7 8 Number of samples 2 2 2 2 Number of non-GM seeds 2000 1992 1984 1992 Number of GM seeds 0 8 16 448 TABLE 1 PT20 sample details Technical Committee Coordinator ISTA Secretariat 8303 Bassersdorf Switzerland nadine.ettelista.ch Nadine Ettel 41SEED TESTING INTERNATIONAL OCTOBER 2015 Subsampling quantification number Results reported in number number Results reported in mass mass Results reported in any other unit such as number DNA copies median of the results Important Note Before the last GMO PT round PT19 a change in the procedure was made in order to better take into account recognized standards regarding the precision of a quantitative result. Therefore in the definition of the Reference intra-laboratory standard deviation used in the computation of the z scores we introduced the following change using the same notations as in the reference document in ISTA News Bulletin No. 130 If reference lab stdevtrue level is lower than 0.25 use 0.25 x true level as the Reference intra-laboratory standard deviation. The ratings awarded in this round were as follows Rate Number of laboratories A 8 B C 3 BMP 1 The reporting units used by participants were as follows Reporting unit Number of laboratories number 6 mass 4 DNA copies 2 For a more comprehensive explanation of the rating system please refer to Seed Testing International No. 130 quantitative rating and No. 128 qualitative rating or contact the ISTA Secretariat. Rating Misclassified samples Misclassified samples absolute numbers Number of laboratories A 05 0 19 B 510 C 1020 1 1 BMP 20 1 1 COMPARISON OF THE THREE QUALITATIVE RATING SYSTEMS PT20 QUALITATIVE RATING Score Classes RS1 RS2 RS3 A B C BMP 20 18 16 14 12 10 8 6 4 2 0 RS1 RS2 RS3 8 oflabs 7 6 5 4 3 2 1 0 A B C BMP 42 SEED TESTING INTERNATIONAL www.seedtest.org Accreditation of the FASMAC Seed Testing Laboratory Kanagawa Japan Satoshi Futo FASMAC Co. Ltd. Genetic Analysis Division Atsugi City Kanagawa Japan gmofasmac.co.jp ACCREDITATION FASMAC CO. LTD. FASMAC located in Kanagawa Japan is a Japan-based biotechnology and food analysis company incorporated in 2001. FASMAC currently provides a number of services including DNA sequencing and gene synthesis and food analysis using the method of molecular biotechnology. Since its establishment FASMACs business in food analysis by the Genetic Analysis Division has offered a wide range of genetic testing services including inspection for genetically modified organisms GMO. This GM testing method for food was also applied to seeds. However our thoughts about the necessity of specific testing methods for seeds led to the development of more advanced GM testing methods. In order to report accurate results it was necessary to improve our quality management system in accordance with international standards. In January 2015 the laboratory received accreditation from the International Seed Testing Association ISTA. It took three years for Genetic Analysis Divisions Seed Laboratory to achieve this through extensive work on improvement of GMO testing methods participation in ISTA proficiency tests validation under the ISTA PDE and FASMACs SOP and training at previously accredited laboratories in Japan. We have developed our skills and experience as a testing laboratory for the inspection of seeds and seedlings. We have developed our skills and experience as a testing laboratory for the inspection of seeds and seedlings. We intend to will expand our scope of ISTA accreditation to seed health testing and germination testing. We will do our best for the safe and secure distribution of seeds by providing high- quality seed analysis services. 43SEED TESTING INTERNATIONAL OCTOBER 2015 Laboratory Accreditation Changes Newly Accredited ISTA Member Laboratories Status 16.03.2015-15.08.15 Re-accredited ISTA Member Laboratories Status 16.03.2015-15.08.15 CONGRATULATIONS ON YOUR ACCREDITATION AND RE-ACCREDITATION PH03 DuPont Pioneer Asia Pacific Regional Quality Laboratory Luisita Industrial Park San Miguel 2301 Tarlac PHILIPPINES Phone 63 45 985 0026 63 91 7592 0342 APRegionalLabSupportpioneer.com AU06 Tasmanian Seed Laboratory Department of Primary Industry Water Environment 165 Westbury Rd Prospect 7250 P.O. Box 46 Kings Meadows 7249 Tasmania AUSTRALIA Phone 61 3 63365248 Fax 61 3 63444961 Ashlee.OCallaghandpipwe.tas. gov.au CH01 Agroscope Forschungsgruppe Saat- gutqualitt Reckenholzstrasse 191 8046 Zrich SWITZERLAND Phone 41 44 3777111 Fax 41 44 3777201 saatgutpruefungart.admin.ch CZ03 Central Institute for Supervising and Testing in Agriculture Department of Seed Testing Za opravnou 4 150 06 Praha 5 - Motol CZECH REPUBLIC Phone 420 2 57211751 Fax 420 257211748 barbora.dobiasovaukzuz.cz DE04 Landwirtschaftliches Technologiezentrum Augustenberg LTZ Referat 23 Saatgutuntersuchung und Angewandte Botanik Nesslerstrasse 23-31 76227 Karlsruhe GERMANY Phone 49 721 9468150 Fax 49 7219468387 andrea.jonitzltz.bwl.de DE05 Landesbetrieb Hessisches Landeslabor LHL Am Versuchsfeld 11-13 34128 Kassel GERMANY Phone 49 561 9888 0 Fax 49 5619888300 monika.hohmannlhl.hessen.de DE18 Thringer Landesanstalt fr Land- wirtschaft TLL Referat Saatgut Naumburger Strasse 98 07743 Jena GERMANY Phone 49 3641 683358 Fax 49 3641426224 guenter.muellertll.thueringen.de DK09 Eurofins Steins Laboratory AS Seed Testing Ladelundvej 85 6600 Vejen DENMARK Phone 45 7022 4286 dstjeurofins.dk FR06 Syngenta Seeds SAS La Gragnette 2 32220 Lombez FRANCE Phone 33 5 6262 6024 Fax 33 5 6262 5511 nathalie.danezansyngenta.com ID02 PT East West Seed Indonesia Quality Assurance Campaka Kabupaten Purwakarta Desa Benteng P.O. Box 1 41181 West Java INDONESIA Phone 62 264 201 871 Fax 62 264 201 875 officepanahmerah.id IT03 CRA Consiglio per la Ricerca in Agricoltu- ra e lAnalisi dellEconomia Agraria CRA-SCS Sede di Tavazzano-Laboratorio Via Emilia km 307 26838 Tavazzano LO ITALY Phone 39 0371 761919 Fax 39 0371760812 scs.loentecra.it IT06 Centro Nazionale per lo Studio e la Con- servazione della Biodiversit Forestale Via del Ponte 256 37020 Peri Verona ITALY Phone 39 045 628 4071 Fax 39 045 628 4089 fabio.gorianistalibero.it KE01 KEPHIS Seed Testing Laboratory P.O. Box 1679 20100 Nakuru KENYA Phone 254 722209503 Fax 254 20 3536170 kephisnakurukephis.org KG01 Republican Seed Inspection RSI Central Seed Laboratory 4-A Toktonaliev Street Bishkek 720055 KYRGYZSTAN Phone 7996 312 542225 Fax 7996 312 540423 kgml01mail.ru LV01 National Seed Testing Laboratory Lielvardes str. 3638 1006 Riga LATVIA Phone 371 711 2716 Fax 371 711 3085 velta.evelonevaad.gov.lv TH03 East West Seed Company Limited Quality Assurance 501 Moo 2 Sainoi-Bang Bua Thong Road T. Sainoi A. Sainoi Nonthaburi 11150 THAILAND Phone 66 2 831 7777 Fax 66 2 597 1229 Tapanee.Attamangkune eastwestseed.com UG02 Chemiphar U Ltd. P.O. Box 25525 Kampala UGANDA Phone 256 0793 42.303 Fax 256 41 268 834 auchemiphar.net 43SEED TESTING INTERNATIONAL OCTOBER 2015 44 SEED TESTING INTERNATIONAL www.seedtest.org Science in ISTA A new initiative Alison Powell ISTA Honorary Life Member Chair ISTA Seed Science Advisory Group School of Biological Sciences University of Aberdeen Aberdeen AB24 3UU United Kingdom a.a.powellabdn.ac.uk SEED SCIENCE SCIENCE IS A KEY COMPONENT of ISTAs work and one of the aims of the ISTA Strategy is to strengthen scientific work within ISTA. This can be achieved by identifying scientific developments with the potential for application in seed testing and initiating co-ordinated development within ISTA laboratories ISTA Strategy 20132016. The recent establishment of the Seed Science Advisory Group SSAG is a crucial step towards achieving this aim. Formation of the Seed Science Advisory Group The SSAG is made up of individuals who have a keen interest in the appropriate application of seed science. The group has 10 voting members four specified in the Terms of Reference and six approved by the ECOM plus two ex officio members table 1 and figure 1. The six approved members were chosen on the basis of them being active applied seed scientists. All ten members have limited involvement with the commercial development of methods for seed quality evaluation. The group had its first meeting on 13 July 2015 during which we discussed the objectives of the group as identified in the Terms of Reference approved by the ECOM and how we would aim to achieve these. The three aims of the SSAG and our plans to achieve these are 1. To provide a link between fundamental research and the use of that research to meet the needs of ISTA members. This will include identifying research results that have applicability to ISTA and facilitating collaboration between the researcher and the end user. To achieve this we shall use our reading of research papers and attendance at conferences to identify ideas that have potential for development and application as seed quality tests. Clearly we do not cover all areas of expertise and therefore we hope to be assisted by a few other individuals who have this expertise to help us. Once we have identified interesting work we shall discuss the potential of the work with the appropriate TCOM and whether they might be interested in assisting in its development. We would then contact the scientists involved and discuss if they would like to progress the work towards application and how best this might be done. This might be through providing guidance to the scientists about the nature of information that is needed for development of a seed quality test or the involvement of a TCOM in its development. 2. Appraisal of evidence for techniques equipment available for use in seed testing laboratories a Identification of and evaluation of novel developments for evaluation New equipment and techniques are frequently developed for which there is limited independent evaluation of their efficacy. The TCOMs and ISTA members who wish to have a new development piece of equipment evaluated can submit a request to the SSAG to evaluate whether it is fit for purpose. The SSAG will also make themselves aware of novel developments. A procedure for the evaluation of proceduresequipment has been outlined and includes gathering together evidence and proof of effectiveness of these developments. The evidence will come from both published papers and formal requests to developers for supplementary evidence and proof of effectiveness. This evidence will be evaluated and a report produced for the ECOM and then published in Seed Testing International. b Development of guidelines to assess equipment and assist in appropriate development of seed quality tests. Guidelines will be developed for the use of ISTA members to assist them in the evaluation of equipment and quality tests in the absence of a report from the SSAG and any ISTA validation. 3. Respond to requests from ECOM and TCOM Chairs for advice related to scientific issues. The ECOM and TCOMs can consult the SSAG on any scientific issues that concern them. Where we lack the expertise on a topic we shall identify someone appropriate to respond. However we want to emphasise that we shall welcome input from ISTA members regarding the development and availability of new methods of seed testing. 45SEED TESTING INTERNATIONAL OCTOBER 2015 Communication We are particularly keen to maintain good lines of communication between ourselves and the ECOM TCOMs and ISTA members. This article and the SSAG page on the ISTA website are the first step and aim to inform everyone about how we plan to operate so that we are as transparent as possible about our aims and how we shall achieve these. The page on the ISTA website also lists the group members their research and any commercial interests. In addition we have given details of the procedures that we will follow when developing potential new ideas with TCOMs and when evaluating new equipment or procedures. We shall be providing the ECOM with a report every six months in addition to any equipment evaluation reports and will maintain email contact with TCOM chairs and report to them at ECOM TCOM meetings. Our contact with ISTA members will largely be at Annual Meetings where we will give a presentation during the technical sessions as well as having an open meeting. However we want to emphasise that we shall welcome input from ISTA members regarding the development and availability of new methods of seed testing. If you become aware of research publications that suggest the potential for development of new methods of seed testing or would like to see equipment that replaces current testing methods evaluated please contact the Chair of the SSAG Alison Powell a.a.powellabdn. ac.uk. We know that you may see developments that we do not We have identified targets for the SSAG to achieve by December 2015 when we shall have a web-conference but have plans for regular e-mail contact in the interim. FIGURE 1. Members of the Seed Science Advisory Group at the first group meeting at SASA Edinburgh on 13 July 2015. Left to right Laura Bowden Jol Lchapp Fiona Hay Alison Powell Jane Thomas Birte Boelt Franoise Corbineau Stan Matthews. Members Noted in the Terms of Reference Alison Powell Chair ISTA Honorary Member University of Aberdeen UK Beni Kaufman ISTA Secretary General Zurich Switzerland Fiona Hay Chief Editor SST. IRRI The Philippines Laura Bowden Seed Symposium Convenor SASA Edinburgh UK Members Approved by the ECOM Birte Boelt Aarhus University Denmark Franoise Corbineau Universit Pierre et Marie Curie Paris France Brigitte Hamman Vice Chair Syngenta Seed Care Basle Switzerland Stan Matthews University of Aberdeen UK Alan Taylor Cornell University USA Jane Thomas NIAB Cambridge UK Ex Officio Members Jol Lchapp ISTA President GEVES Angers France Steve Jones ISTA ECOM Member Chair ISTA Rules Committee CFIA Canada TABLE 1. Members of the Seed Science Advisory Group In absentia Brigitte Hamman Steve Jones Beni Kaufman Alan Taylor 46 SEED TESTING INTERNATIONAL www.seedtest.org TRAINING AND EDUCATION ANNOUNCEMENTS ISTA Quality Assurance Workshop for Advanced Laboratories SakuEstonia10-13June2016 Organizer Agricultural Research Centre Estonian Seed Testing Laboratory Teaduse 46 75501 Saku EE-Estonia Contact Person Heidi Kollo Phone 372 6729118 Mail heidi.kollopmk.agri.ee Lecturers Ronald Don ISTA Technical Auditor ISTA Honorary Life Member Member of several ISTA Technical Committees Florina Palada ISTA System Auditor Aim of the Workshop To give experienced laboratories the opportunity to discuss and obtain inspiration on how to improve their quality assurance system and benefit from it. Target Groups The workshop is for members of experienced laboratories that have already a well-running quality management system in place. The lecturers will focus on selected topics to be discussed. Content The workshop will consist of oral presentations group work and exercises. The lecturers will try to involve the participants as much as possible. Participants are invited to bring their laptops along. Some of the workshop content will deal with General management technical aspects Statistical aspects of seed testing Calibrationverification of equipment etc. How to read uncertainty on calibration certificates Internal quality control Check testing blind tests etc. Analysis Trends Monitoring of staff seed samplers and analysts How to deal with non-conformities requirements of selected topics of the Accreditation Standard The workshop will include visit of the ISTA accredited Estonian Seed Testing Laboratory. Location Agricultural Research Centre Estonian Seed Testing Laboratory Teaduse 46 75501 Saku EE-Estonia Phone 372 6517670 Fax 372 6562643 Accommodation List of affiliated hotels currently for general information only Original Sokos Hotel Viru Solo Sokos Hotel Estoria Hotel Palace Tallinn Radisson Blu Sky Hotel Savoy Boutique Hotel The participants are asked to book the hotels directly. Transportation from the hotels to the workshop venue will be organized. Registration Fee Details will be published on the ISTA website soon. The registration fee will include all literature and supporting material for the workshop lunches and coffee breaks excursion workshop dinner and transfer between the workshop venue and hotels except airport transfer. It does not include accommodation or meals other than those specified. Registration Information will be published soon on the ISTA website www.seedtest.orgen workshop.html 47SEED TESTING INTERNATIONAL OCTOBER 2015 ISTA Workshop on Germination Testing SakuEstonia10-13June2016 Organizer Agricultural Research Centre Estonian Seed Testing Laboratory Teaduse 46 75501 Saku EE-Estonia Contact Person Heidi Kollo Phone 372 6729118 Mail heidi.kollopmk.agri.ee Lecturers Sylvie Ducournau Germination Committee Chair Another member of the Germination Committee tbc Aim of the Workshop This workshop aims at presenting and discussing the principles of the ISTA germination test seedling evaluations for different species results calculations and reporting plus aspects of quality management linked to germination testing. Target Groups The workshop is for persons who are involved in seed testing according to the ISTA Rules. Content The workshop will consist of lectures and practical exercises. It will offer the opportunity for general discussion on germination testing as well as for specific questions regarding testing procedures. The ISTA Rules and the ISTA Handbooks will be discussed and used during the workshop. Participants of this workshop will be actively involved through group work discussions and practical work. Language of the Workshop is English. Preliminary Programme Introduction to ISTA Current and future work of the ISTA germination committee Testing procedures for germination Seedling evaluation Calculations and tolerances Quality assurance aspects Preliminary list of species for the Workshop Grass seeds Lolium spp. Festuca spp. Poa spp. Forage crops Medicago sp. Trifolium sp. Pisum Lupinus Vicia faba Oil seeds Helianthus annus Vegetable seeds Brassica Allium Daucus The workshop will include visit of the ISTA accredited Estonian Seed Testing Laboratory. Location Agricultural Research Centre Estonian Seed Testing Laboratory Teaduse 46 75501 Saku EE-Estonia Accommodation Details will be published on the ISTA website soon. The participants are asked to book the hotels directly. Transportation from the hotels to the workshop venue will be organized. Registration Fee Details will be published on the ISTA website soon. The registration fee will include all literature and supporting material for the workshop lunches and coffee breaks excursion workshop dinner and transfer between the workshop venue and hotels except airport transfer. It does not include accommodation or meals other than those specified. Registration Information will be published soon on the ISTA website www.seedtest.orgen workshop.html 48 SEED TESTING INTERNATIONAL www.seedtest.org WORKSHOP REPORTS ISTA Workshop on Seed Sampling and Quality Assurance in Seed Sampling ColoniaUruguay9-12June2015 Max Soepboer Former Vice-Chair of the ISTA Bulking and Sampling Committee 6871 EG Renkum Netherlands maxsconsultgmail.com IN JUNE 2014 the Instituto Nacional de Semillas INASE of Uruguay hosted the first ISTA seed sampling workshop ever held in Latin America. INASE has its headquarters at Canelones near Colonia. The laboratory of INASE which is ISTA accredited is also located here. There were 27 participants from Argentina Bolivia Brazil Colombia Costa Rica United States Peru and Uruguay. Because of the high number of Spanish- speaking participants INASE had contracted two interpreters who did simultaneous translation into Spanish and vice versa. This translation contributed highly to a better understanding of the lectures and at the same time it stimulated the discussions. The lecturers for the workshop were Leena Pietil Vice-Chair of the Bulking and Sampling Committee BSC Eddie Goldschagg Chair of the BSC and Max Soepboer former Vice-Chair of the BSC. All three have been members of the BSC and have previously lectured at various sampling workshops. The workshop started with a short introduction round of the participants and the lecturers. After that the participants were asked what they expected from the workshop and these expectations were listed. The workshop focused on two topics seed sampling and quality assurance in seed sampling. Both were dealt with theoretically and in practice. The theoretical lessons were held in the meeting room of INASE and the practicals took place in the INASE warehouse where seed is stored in different types of containers. With regard to seed sampling the following aspects were covered General principles of seed sampling Introduction to sampling methods Seed sampling sealing and labelling of seed lots General principles and types of automatic seed samplers 49SEED TESTING INTERNATIONAL OCTOBER 2015 ISTA protocol for approval of automatic samplers Testing and approval of automatic seed samplers Methods for reduction of the composite sample Training examination and authorisation of seed samplers Concerning quality assurance in seed sampling the following items were dealt with Check of sample dividers The ISTA Accreditation Standard Quality assurance in seed sampling Internal quality control in seed sampling Monitoring of seed samplers Internal audits and assessments Non-conformities and corrective actions Each item was covered by a presentation of which the participants received hand-outs. Participants were invited not only to ask questions but also to present their working methods and share experiences with each other. During the practicals on seed sampling participants practised various ways of sampling such as sampling of bags with the Nobbe trier sampling of big bags with the sampling stick and with the extended Nobbe trier and sampling of small containers. Besides this participants carried out various sample homogenization and reduction methods with the Riffle divider rotary divider and the hand-halving method. The calibration of these three types of dividing equipment was also practised. For this a mixture of wheat and soybean seed was used. The results of these calibrations were scored and checked whether they were within tolerance. The practicals on quality assurance in seed sampling consisted of Drafting an instruction for sampling Defining the root cause for a non- conformity and drafting a plan to prevent the same non-conformity in the future and Classifying a number of non-conformities into major or minor. For these assignments the group was divided into small subgroups that each had to work out the assignment. The results of the work of the subgroups were subsequently discussed in the plenary group. For Wednesday afternoon INASE had organised a visit to Greising y Elizarz SRL TararirasColonia a seed company that multiplies processes and sells seed of fodder crops both legumes and grasses cereals and soybeans. At the end of the afternoon there was a guided tour of the old city centre of Colonia del Sacramento. The guide explained that three Sampling of big bags. Practising the hand-halving method. hundred years ago the city was contested by Portugal and Spain because of its strategic position. As a result Colonia changed hands many times between these two countries. The architecture and cobblestone streets of the historic city centre reflect the periods of Portuguese and Spanish rule. On Thursday evening the official workshop dinner was held in Pulperia de Los Faroles an authentic and convivial restaurant in the old city of Colonia del Sacramento. At the end of the workshop the list of expectations was evaluated and it was concluded that the expectations as expressed at the beginning of the workshop had been met. The general feeling of the participants was that the workshop was valuable informative and supportive for their daily work at home. The workshop was concluded with the presentation of the certificates to the participants. The old city centre of Colonia del Sacramento. 50 SEED TESTING INTERNATIONAL www.seedtest.org WORKSHOP REPORTS ISTA Workshop on Tetrazolium Testing for Viability and its use as a Vigour Test for Glycine max ColoniadelSacramentoUruguay9-12 June2015 Jos Frana Neto President Associao Brasileira de Tecnologia de Sementes ABRATES Empresa Brasileira de Pesquisa Agropecuria Embrapa Parque Estao Biolgica sn Braslia DF CEP 70770-901 Brazil jose.francaembrapa.br THE ISTA TETRAZOLIUM COMMITTEE the Vigour Committee and the National Seed Institute INASE Uruguay organized and promoted the Workshop on tetrazolium testing for viability and its use as a vigour test for Glycine max from 9-12 June 2015. The workshop took place in the La Estanzuela Experimental Station of the National Agricultural Research Institute INIA in the city of Colonia del Sacramento Uruguay. The workshop was supported by the National Agricultural Technology Institute INTA Argentina Embrapa Soybean and the Brazilian Association of Seed Technologists ABRATES Brazil. Carina Gallo INTA Oliveros Argentina Teresita Farrs and Vanessa Sosa INASE Participants and instructors of the workshop. 51SEED TESTING INTERNATIONAL OCTOBER 2015 Uruguay and Silvana Gonzalez INIA Uruguay headed the organization of the workshop with the participation of Roque Craviotto Miriam Arango and Marcelo Fared INTA Oliveros Argentina and Jos Frana-Neto Embrapa SoybeanABRATES Brazil as instructors and the technical support of Vivina Prez and Laura Telechea INASE Uruguay. Seventeen seed specialists participated in the workshop 16 from Uruguay and one from Chile. The workshop was made up of lectures interactive sessions and practical experience in tetrazolium testing. This included its use for determining the vigour of soybean seeds a vigour test that was recently validated by ISTA. It also offered the opportunity for general discussion of these techniques and provided time for participants to ask specific questions regarding tetrazolium and vigour test methods and procedures. With the knowledge gained participants can now sort the seeds into different levels of viability and vigour as determined by the tetrazolium test and can predict the quality of the seeds based on these two aspects. Instructors and organizers top left to right Silvana Gonzalez INIA Uruguay Miriam Arango INTA Oliveros Argentina Teresita Farrs and Vanessa Sosa INASE Uruguay bottom left to right Roque Craviotto INTA Oliveros Argentina and Jos Frana Neto Embrapa Soybean ABRATES Brazil. General view of the workshop. 52 SEED TESTING INTERNATIONAL www.seedtest.org WORKSHOP REPORTS Senior Seminar Seed Testing and Seed Quality Control 5-6 May 2015 Zhejiang University Hangzhou China Prof. Dr. Norbert Leist 76669 Bad SchnbornMingolsheim Germany norbert.leistpartner.kit.edu IT WAS IN 1996 when the former director Professor Yan inquired at the ISTA Secretariat about a workshop on seed testing methods. The result was a two-week workshop in Hangzhou on all the topics of seed testing held by Richard Coster UK and Norbert Leist Germany with 60 participants. Since then the exchange between scientists has been growing and numerous seminars and workshops have taken place. In light of this cooperation and in order to further improve seed scientific theories and increase the knowledge of researchers and teaching staff as well as to improve the seed quality control conducted by seed enterprises the senior seminar on Seed Testing and Seed Quality Control took place on 5th and 6th May 2015. The seminar was held at the Zhejiang University Zijingang Campus and was attended by about 50 participants from various universities research institutes and seed companies from all over China. The German expert and former ISTA President Prof. Norbert Leist and the Director of the Seed Testing Division of the National Agricultural Technology Promotion Center Ministry of Agriculture Juzhen Zhi were invited to give lectures. Prof. Leist introduced the latest revisions of the International Rules for Seed Testing from the past five years and the progress of seed testing technology by ISTA. Director Juzhen Zhi presented the current situation of seed quality in China and the new dynamics of laws and regulations involving seed testing. The new technology of seed testing administration of seed quality and the future development of national seed testing were illustrated further. Professor Jin Hu Chair of the Agronomy Department and Head of the Seed Science Center of Zhejiang University introduced the new methods of seed vigor testing and the progress in research on seed science and technology. The agenda was as follows 1. The seed world and the International Seed Testing Association. N. Leist 2. Seed Testing and Seed Quality Control in China. Juzhen Zhi 3. The ISTA Accreditation System. N. Leist 4. GMO the ISTA performance based approach and thresholds. N. Leist 5. Activities in ISTA Technical Committees. N. Leist 6. Sampling from the seed lot and in the laboratory. N. Leist 7. Tetrazolium for testing viability and vigor together. N. Leist 8. ISTA methods for seed health testing. N. Leist 9. Some principles of Quality Assurance. N. Leist 10. New changes of ISTA seed testing rules. N. Leist 11. Seed vigour and its new testing methods. Jin Hu 12. Advances in seed science and technology. Jin Hu The seminar was organized by the Seed Science Center College of Agriculture and Biotechnology of Zhejiang University and supported by Dr. Andrea Jonitz Head of the Seed Testing Station Augustenberg Germany and by the ISTA Secretariat Switzerland. The lively discussion following the presentations and during the coffee breaks showed a high level of expertise and interest. Since staff from seed testing laboratories were also present the lectures concerning accreditation were of high topicality. At the end all participants were familiar not only with sampling and testing methods but also with the goals and means of ISTA. The interest in ISTA membership was noticeable. In general the seminar was said to be of great value to the Chinese seed testing laboratories and institutions and that further activities in this field should be encouraged. EMBRACE ISTAS VISION OF UNIFORMITY IN SEED TESTING Founded in 1924 as an international non-profit association ISTA operates under the governments of its member countries. ISTAs work is endorsed by international organizations such as the ISF UPOV and the WTO it guarantees worldwide uniformity in seed testing. 1 FOLLOW THE INTERNATIONAL RULES FOR SEED TESTING Annually updated the ISTA Rules provide standardized methods to be used in sampling testing seed lot quality and reporting results internationally.2 BENEFIT FROM THE KNOWLEDGE OF ISTAS TECHNICAL COMMITTEES ISTA counts 18 Technical Committees whose work is to solve technical challenges that in most cases are not unique to one company. They represent the most efficient and cost-effective way of addressing analytical needs. 3 EVALUATE YOUR PERFORMANCE WITH THE PROFICIENCY TEST PROGRAMME The Proficiency Test programme is exclusive to ISTA. It provides an opportunity for seed laboratories to assess their technical performance and to benchmark themselves against the ISTA laboratory community.4 WITH ISTA ACCREDITATION OBTAIN RECOGNITION OF YOUR COMPETENCE There are more than 130 ISTA accredited laboratories globally. Accredited laboratories have proven their technical competence in carrying out seed testing in accordance with the ISTA Rules and operate an effective quality management system. 5 ISSUE ISTA CERTIFICATES TO GROW YOUR BUSINESS INTERNATIONALLY Once accredited laboratories are authorized to issue ISTA Certificates. ISTA Certificates create a common language for seed vendors and buyers benefiting companies wanting to expand sales internationally.6 MAKE YOUR VOICE HEARD ISTA recently introduced the category of Industry Membership. Aiming at further engaging with the seed industry it is meant for all sizes of companies and provides a forum to voice the seed industrys concerns within ISTA. 7 JOIN A GLOBAL NETWORK OF SEED SCIENCE PROFESSIONALS ISTAs community includes more than 300 seed testing laboratories and individual seed professionals in 80 countries. ISTA is a global network of experts to whom to turn to for all seed testing challenges.8 STAY UP TO DATE READ ISTA PUBLICATIONS ISTA publishes a range of technical handbooks the scientific journal Seed Science and Technology the semi-annual news bulletin Seed Testing International and proceedings from ISTA events. Stay informed9 ACQUIRE KNOWLEDGE IN ISTA WORKSHOPS AND EVENTS ISTA regularly holds workshops and events which provide a platform for acquiring and exchanging informa- tion experience and ideas. ISTA Members benefit from prioritized and reduced registration fees.10 www.seedtest.org Follow ISTA on 10very good reasons to join ISTA International Seed Testing Association ISTAInternational Seed Testing Association ISTA The 31st ISTA Congress will be held in Tallinn Estonia June 15-21 2016 SAVE THESE DATES ISTA Seed Symposium theme for 2016 Progress in seed testing and seed quality improvement through science and technology A limited number of exhibition stands are available. For further information about the programme or sponsorship opportunities please visit www.ista-tallinn2016.ee Follow ISTA on WE LOOK FORWARD TO WELCOMING YOU IN ESTONIA